NEBNext® Quick DNA Library Prep Reagent Set for 454™

E6090 can be substituted for this application
  • This product was discontinued on 03/18/2014
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Description

The NEBNext® Quick DNA Library Prep Reagent Set for 454™ contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the 454 platform.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBuffer 2-2010X
Deoxynucleotide Solution Mix-2010 mM
T4 DNA Polymerase-203,000 units/ml
Adenosine-5'-Triphosphate (ATP)-2010 mM
T4 Polynucleotide Kinase-2010,000 units/ml
Taq DNA Polymerase with ThermoPol® Buffer-205,000 units/ml
Quick T4 DNA Ligase-20
NEBNext Sizing Buffer1.2X
TE Buffer

Properties and Usage

Materials Required but not Supplied

Agencourt® Ampure® Beads (Beckman Coulter)
DNA Adaptors or Vectors
Magnetic Separation Rack (NEB #S1510)

Storage Temperature

-20°C

Protocols

  1. NEBNext End Repair and dA-Tailing (E6080)
  2. Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
  3. NEBNext Adaptor Ligation (E6080)
  4. NEBNext Small Fragment Removal (E6080)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.