NEBNext® DNA Library Prep Master Mix Set for 454™
The NEBNext® DNA Library Prep Master Mix Set for 454™ contains reagents for preparation of DNA libraries for next-generation sequencing. Please note that adaptors and primers are not included in the kit.
The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
The following reagents are supplied with this product:
Store at (°C) Concentration Bst DNA Polymerase, Large Fragment -20 Molecular Biology Grade Water -20 Hydrophilic Streptavidin Magnetic Beads 4 4 mg/ml NEBNext Bead Wash Buffer 4 1 X NEBNext Bead Binding Buffer 4 2 X NEBNext Adapter Fill-in Reaction Buffer -20 10 X NEBNext End Repair Enzyme Mix -20 NEBNext End Repair Reaction Buffer -20 10 X Quick T4 DNA Ligase -20 NEBNext Quick Ligation Reaction Buffer -20 5 X
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