The NEBNext® DNA Library Prep Master Mix Set for 454™ contains reagents for preparation of DNA libraries for next-generation sequencing. Please note that adaptors and primers are not included in the kit.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Bst DNA Polymerase, Large Fragment|
|Molecular Biology Grade Water|
|Hydrophilic Streptavidin Magnetic Beads||4 mg/ml|
|NEBNext Bead Wash Buffer||1 X|
|NEBNext Bead Binding Buffer||2 X|
|NEBNext Adapter Fill-in Reaction Buffer||10 X|
|NEBNext End Repair Enzyme Mix|
|NEBNext End Repair Reaction Buffer||10 X|
|Quick T4 DNA Ligase||-20|
|NEBNext Quick Ligation Reaction Buffer||-20||5 X|
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