NEBNext® DNA Library Prep Set for SOLiD™

  • This product was discontinued on 02/06/2014
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Discontinued Products


The NEBNext® DNA Library Prep Set for SOLiD™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

Functional Validation
Each set of reagents is functionally validated together through construction and sequencing of a DNA library on the SOLiD™ 4.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
NEBNext End Repair Reaction Buffer10X
T4 DNA Ligase
NEBNext Quick Ligation Reaction Buffer-205X
LongAmp™ Taq 2X Master Mix-202X
NEBNext End Repair Enzyme Mix-20

Properties and Usage

Storage Temperature



  1. What type of sample prep/library construction can I use the NEBNex®t DNA Library Prep Set for SOLiD™ reagents for?
  2. What type and how much starting material do I need to use when preparing libraries using the NEBNext® DNA Library Prep Set for SOLiD™?
  3. Can I use the NEBNext® DNA Library Prep Set for SOLiD™ reagents for preparing RNA miRNA work?
  4. Can NEB provide any of the reagents for emulsion PCR step or the actual SOLiD™ sequencing run?
  5. Are these reagents available in a customized format, larger format or 96 well format?


  1. NEBNext End Repair Module Protocol (E6060)
  2. NEBNext Quick Ligation Module Protocol (E6060)
  3. LongAmp™ Taq 2X Master Mix Protocol (E6060)


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.