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  • NEBNext® DNA Library Prep Reagent Set for 454™

    Replaced by E6070
    • This product was discontinued on 09/08/2009
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    Discontinued Products


    Refer to your specific sample preparation protocol to determine conditions for use.

    The NEBNext DNA Library Prep Reagent Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing (1), and for preparation of single stranded DNA for use in high density hybridization arrays (2) or for genomic subtraction hybridization methods (3). Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.

    For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact nebsolutions@neb.com for further information.

    Functional Validation

    Each set of reagents is functionally validated together through construction and sequencing of a transcriptome library and sequenced on a , .

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Properties and Usage

    Storage Temperature



    1. Straus, D. and F.M. Ausubel (1990). Genomic subtraction for cloning DNA corresponding to deletion mutation. Proc. Natl. Acad. Sci. USA. 87, 1889-1893.
    2. Zhou, X. and D.T. Wong (2007). Single nucleotide polymorphism mapping array assay. Methods Mol. Biol.. 396, 295-314.
    3. Maricic, T. and S. Paabo (2009). Optimization of 454 sequencing library preparation from small amounts of DNA permits sequence determination of both DNA strands. Biotechniques. 46, 51-52, 54-57. PubMedID: 3422


    1. Do you provide a protocol for using the NEBNext DNA Library Prep Reagent Set for 454 reagents?
    2. What type of sample prep/library construction can I use the NEBNext DNA Library Prep Reagent Set for 454 reagents for?
    3. What type and how much starting material do I need to use when preparing libraries using the NEBNext DNA Library Prep Reagent Set for 454?
    4. Can I use these reagents for preparing RNA? For miRNA work?
    5. Can I use these reagents for preparing libraries for sequencing on the Illumina GA II?
    6. Can I use these reagents for preparing libraries for sequencing on the ABI SOLiD instrument?
    7. Are these reagents available in a customized format? Larger format or 96 well format?
    8. Will use of these reagents void the warranty on my 454 instrument?


    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

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    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.