• My NEB
  • Print
  • PDF
  • E. coli K12 ER2420/pBeloBAC11

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4154S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    A suspension of E. coli strain ER2420 containing the plasmid pBeloBAC11 which has been grown in rich medium and brought to 50% glycerol. ER2420 background: EcoK r- m- McrBC- Dam+ Dcm+.

    Genotype

    F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101

    Properties and Usage

    Storage Temperature

    -20°C

    Growth Media

    LB

    Growth Temperature

    37 °C

    Growth Medium and Conditions

    The plasmid pBeloBAC11 carries a gene encoding resistance to chloramphenicol (cam). To maintain the plasmid, cells should be grown with 15-20 µg/ml cam.

    Notes

    1. The blue color of non-recombinant pBeloBac11 clones on indicator plates is not as intense as that obtained with multicopy vectors that also use α-complementation of β-galactosidase. We recommend incubation for 20-24 hours at 37°C to achieve the full color development necessary to evaluate transformation efficiency and guide selection of white colonies. Color development will intensify further if the plates are subsequently stored at 4°C.

    References

    1. Shizuya, H. et al. (1992). Proc. Natl. Acad. Sci. USA. 89, 8794-8797.
    2. Mori, H. et al. (1986). J. Mol. Biol.. 192, 1-15.
    3. Imber, R., Low, R.L. and Ray, D.S. (1983). Proc. Natl. Acad. Sci. USA. 80, 7132-7136.
    4. Disque-Kochem, C. et al. (1986). Mol. Gen. Genet.. 202, 132-135.
    5. Komori, H. et al. (1999). EMBO J.. 18, 45974607.
    6. Lane, D. et al. (1986). Nucl. Acids Res.. 14, 9713-9728.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.