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  • E. coli K12 ER2420/pBeloBAC11

    Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli strain ER2420 containing the plasmid pBeloBAC11 which has been grown in rich medium and brought to 50% glycerol. ER2420 background: EcoK r- m- McrBC- Dam+ Dcm+.

    Genotype

    F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101

    Properties and Usage

    Storage Temperature

    -20°C

    Growth Media

    LB

    Growth Temperature

    37 °C

    Growth Medium and Conditions

    The plasmid pBeloBAC11 carries a gene encoding resistance to chloramphenicol (cam). To maintain the plasmid, cells should be grown with 15-20 µg/ml cam.

    Notes

    1. The blue color of non-recombinant pBeloBac11 clones on indicator plates is not as intense as that obtained with multicopy vectors that also use α-complementation of β-galactosidase. We recommend incubation for 20-24 hours at 37°C to achieve the full color development necessary to evaluate transformation efficiency and guide selection of white colonies. Color development will intensify further if the plates are subsequently stored at 4°C.

    References

    1. Shizuya, H. et al. (1992). Proc. Natl. Acad. Sci. USA. 89, 8794-8797.
    2. Mori, H. et al. (1986). J. Mol. Biol.. 192, 1-15.
    3. Imber, R., Low, R.L. and Ray, D.S. (1983). Proc. Natl. Acad. Sci. USA. 80, 7132-7136.
    4. Disque-Kochem, C. et al. (1986). Mol. Gen. Genet.. 202, 132-135.
    5. Komori, H. et al. (1999). EMBO J.. 18, 45974607.
    6. Lane, D. et al. (1986). Nucl. Acids Res.. 14, 9713-9728.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.