E. coli K12 POP2136/pFOS1

Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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Strain Products

Description

A suspension of E. coli strain POP2136 containing the plasmid pFOS1 which has been grown in rich medium and brought to 50% glycerol. POP2136 background: EcoK r- m+ McrBC+ Dam+ Dcm+.

Genotype

F- glnV44 hsdR17 endA1 thi-1 aroB mal- cI857 lambdaPR

Properties and Usage

Storage Temperature

-20°C

Growth Media

LB

Growth Temperature

30 °C

Growth Medium and Conditions

Grow cells at 30°C. The plasmid is unstable at 37°C. The plasmid pFOS1 carries a gene encoding resistance to ampicillin (amp) and a gene encoding resistance to chloramphenicol (cam). To maintain the plasmid, cells should be grown with 100 µg/ml amp and 15-20 µg/ml cam.

References

  1. Shizuya, H. et al. (1992). Proc. Natl. Acad. Sci. USA. 89, 8794-8797.
  2. Mori, H. et al. (1986). J. Mol. Biol.. 192, 1-15.
  3. Imber, R., Low, R.L. and Ray, D.S. (1983). Proc. Natl. Acad. Sci. USA. 80, 7132-7136.
  4. Disque-Kochem, C. et al. (1986). Mol. Gen. Genet.. 202, 132-135.
  5. Komori, H. et al. (1999). EMBO J.. 18, 4597-4607.
  6. Lane, D. et al. (1986). Nucl. Acids Res.. 14, 9713-9728.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.