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  • E. coli K12 ER2420/pACYC184

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4152S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    A suspension of E. coli strain ER2420 containing the plasmid pACYC184 which has been grown in rich medium and brought to 50% glycerol. ER2420 background: EcoK r- m- McrBC- Mrr- Dam+ Dcm+.

    Genotype

    F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101

    Properties and Usage

    Storage Temperature

    -20°C

    Growth Media

    LB

    Growth Temperature

    37 °C

    Growth Medium and Conditions

    The plasmid pACYC184 carries a gene encoding resistance to tetracycline (tet) and a gene encoding resistance to chloramphenicol (chloramphenicol acetyltransferase). To maintain the plasmid, cells should be grown with 25-50 µg/ml of tet and 35-50 µg/ml of cam. The plasmid, which is present at a low copy number, cannot be amplified using a standard chloramphenicol procedure but can be amplified using spectinomycin (50 µg/ml of growth media).

    References

    1. Chang, A. and Cohen, S. (1978). J. Bacteriol.. 134, 1141-1156.
    2. Rose, R.E. (1988). Nucl. Acids Res.. 16, 355.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.