• My NEB
  • Print
  • PDF
  • E. coli K12 ER2420/pACYC177

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4151S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    A suspension of E. coli strain ER2420 containing the plasmid pACYC177 which has been grown in LB medium and brought to 50% glycerol. ER2420 background: EcoK r- m- /McrBC- Mrr Dcm+.

    Genotype

    F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101

    Properties and Usage

    Storage Temperature

    -20°C

    Growth Media

    LB

    Growth Temperature

    37 °C

    Growth Medium and Conditions

    The plasmid pACYC177 carries a gene encoding resistance to ampicillin (beta-lactamase) and a gene encoding resistance to kanamycin (kanamycin phosphotransferase). To maintain the plasmid cells should be grown with 50-100 µg/ml of amp and 35-50 µg/ml of kanamycin. The plasmid, which is present at a low copy number, should be amplified using a standard chloramphenicol procedure.

    References

    1. Chang, A. and Cohen, S. (1978). J. Bacteriol.. 134, 1141-1156.
    2. Rose, R.E. (1988). Nucl. Acids Res.. 16, 355.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.