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  • E. coli K12 CJ236

    Limit of 1 per order. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli strain CJ236 which has been grown in LB medium and brought to 50% glycerol.

    Genotype

    (HindIII)::cat (Tra+ Pil+ CamR)/ ung-1 relA1 dut-1 thi-1 spoT1 mcrA

    Properties and Usage

    Antibiotic Resistance

    • cam

    Growth Media

    LB

    Growth Temperature

    37 °C

    Growth Medium and Conditions

    1. Streak out the strain on LB agar containing chloramphenicol (15 µg/ml) to ensure that you start with an F+ host.
    2. Do not include chloramphenicol in liquid media: the strain does not grow well.
    3. CJ236 is non-suppressing. Do not use M13 vectors that carry amber mutations (mp8, 9 and some mp10 and 11).
    4. Store plates at 4°C in the dark.

    Notes

    1. CJ236 is dut- ung- and contains an F-factor carrying a selectable marker (chloramphenicol resistance). It was made by taking an isolate of BW313 that had spontaneously become F- and introducing pCJ105, the F´ CmR construct (1,2). For use in preparing uracil-containing M13 templates (3,4).

    References

    1. Joyce, C. and Grindley, N. (1984). J. Bacteriol.. 158, 636-643.
    2. Raleigh, E.A., Lech, K. and Brent, R. (1989). F.M. Ausubel et al.(Ed.), Current Protocols in Molecular Biology. x. New York, Unit 1.4: Publishing Associates and Wiley Interscience.
    3. Kunkel, T.A., Bebenek, K. and McClary, J. (1991). J. Miller(Ed.), Methods Enzymol.. 204, 125-139. San Diego: Academic Press.
    4. Kunkel, T.A. et al. (1987). R. Wu and L. Grossman(Ed.), Methods Enzymol.. 154, 367-382. San Diego: Academic Press.

    FAQs

    1. How is the damaged DNA that you test the PreCR Repair Mix against created? Can I buy this damaged DNA separately?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.