E. coli K12 UT5600

  • Catalog # E4129 was discontinued on March 07, 2018 ;
  • Product Information
    A suspension of E. coli K12 UT5600 which has been grown in LB medium and brought to 50% glycerol. This strain is deficient in the OmpT protease, a periplasmic protease. This protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and it may degrade proteins expressed in the periplasm as well. UT5600 is EcoK r+ m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.

    Genotype

    F- ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1
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