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  • E. coli K12 UT5600

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4129S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    A suspension of E. coli K12 UT5600 which has been grown in LB medium and brought to 50% glycerol. This strain is deficient in the OmpT protease, a periplasmic protease. This protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and it may degrade proteins expressed in the periplasm as well. UT5600 is EcoK r+ m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.

    Genotype

    F- ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • str

    Growth Media

    LB

    Growth Temperature

    37 °C

    References

    1. Elish, M.E., Pierce, J.R. and Earhart, C.F. (1988). J. Gen. Microbiol.. 134, 1355-1364.
    2. Grodberg, J. and Dunn, J. (1988). J. Bacteriol.. 170, 1245-1253.
    3. Sugimura, K. and Higashi, N. (1988). J. Bacteriol.. 170, 3650-3654.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.
    1. When we analyze our fusion protein expression by Western blot using the Anti-MBP Monoclonal Antibody, only a small fraction of the protein is full-length, while most of it migrates close to the MBP5* marker.