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  • E. coli K12 UT5600

    Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products


    A suspension of E. coli K12 UT5600 which has been grown in LB medium and brought to 50% glycerol. This strain is deficient in the OmpT protease, a periplasmic protease. This protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and it may degrade proteins expressed in the periplasm as well. UT5600 is EcoK r+ m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.


    F- ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1

    Properties and Usage

    Storage Temperature


    Antibiotic Resistance

    • str

    Growth Media


    Growth Temperature

    37 °C


    1. Elish, M.E., Pierce, J.R. and Earhart, C.F. (1988). J. Gen. Microbiol.. 134, 1355-1364.
    2. Grodberg, J. and Dunn, J. (1988). J. Bacteriol.. 170, 1245-1253.
    3. Sugimura, K. and Higashi, N. (1988). J. Bacteriol.. 170, 3650-3654.


    1. When we analyze our fusion protein expression by Western blot using the Anti-MBP Monoclonal Antibody, only a small fraction of the protein is full-length, while most of it migrates close to the MBP5* marker.

    Interactive Tools

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.