E. coli K12 UT5600

Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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Strain Products

Description

A suspension of E. coli K12 UT5600 which has been grown in LB medium and brought to 50% glycerol. This strain is deficient in the OmpT protease, a periplasmic protease. This protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and it may degrade proteins expressed in the periplasm as well. UT5600 is EcoK r+ m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.

Genotype

F- ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1

Properties and Usage

Storage Temperature

-20°C

Antibiotic Resistance

  • str

Growth Media

LB

Growth Temperature

37 °C

References

  1. Elish, M.E., Pierce, J.R. and Earhart, C.F. (1988). J. Gen. Microbiol.. 134, 1355-1364.
  2. Grodberg, J. and Dunn, J. (1988). J. Bacteriol.. 170, 1245-1253.
  3. Sugimura, K. and Higashi, N. (1988). J. Bacteriol.. 170, 3650-3654.

FAQs

  1. When we analyze our fusion protein expression by Western blot using the Anti-MBP Monoclonal Antibody, only a small fraction of the protein is full-length, while most of it migrates close to the MBP5* marker.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.