E. coli K12 ER2508

Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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Strain Products

Description

A suspension of E. coli strain ER2508 which has been grown in LB medium and brought to 50% glycerol.

Genotype

F- ara-14 leuB6 fhuA2 Δ(argF-lac)U169 lacY1 lon::miniTn10(TetR) glnV44 galK2 rpsL20(StrR) xyl-5 mtl-5 Δ(malB) zjc::Tn5(KanR) Δ(mcrC-mrr)HB101

Properties and Usage

Storage Temperature

-20°C

Antibiotic Resistance

  • kan
  • tetracycline
  • str

Growth Media

LB

Growth Temperature

37 °C

Notes

  1. This strain is a derivative of RR1 that has been made lon-. The lon mutation knocks out the major ATP-dependent protease in the E. coli cytoplasm, thus improving the stability of many foreign proteins when expressed in E. coli. The malE gene, which encodes Maltose-Binding Protein, is deleted as part of the malB deletion.
  2. This strain replaces PR745. It is fhuA2.

References

  1. Kowit, J.D. and Goldberg, A.L. (1977). J. Biol. Chem.. 252, 8350-8357.
  2. Grossman, A.D., Burgess, W.W. and Gross, C.A. (1983). Cell. 32, 151-159.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.