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  • E. coli K12 ER2508

    Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli strain ER2508 which has been grown in LB medium and brought to 50% glycerol.

    Genotype

    F- ara-14 leuB6 fhuA2 Δ(argF-lac)U169 lacY1 lon::miniTn10(TetR) glnV44 galK2 rpsL20(StrR) xyl-5 mtl-5 Δ(malB) zjc::Tn5(KanR) Δ(mcrC-mrr)HB101

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • kan
    • tetracycline
    • str

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. This strain is a derivative of RR1 that has been made lon-. The lon mutation knocks out the major ATP-dependent protease in the E. coli cytoplasm, thus improving the stability of many foreign proteins when expressed in E. coli. The malE gene, which encodes Maltose-Binding Protein, is deleted as part of the malB deletion.
    2. This strain replaces PR745. It is fhuA2.

    References

    1. Kowit, J.D. and Goldberg, A.L. (1977). J. Biol. Chem.. 252, 8350-8357.
    2. Grossman, A.D., Burgess, W.W. and Gross, C.A. (1983). Cell. 32, 151-159.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.