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  • E. coli K12 PR1031

    Limit of 1 per order. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli K12 strain PR1031 which has been grown in LB medium and brought to 50% glycerol. Grow by streaking out on an LB plate and incubating at 37°C. This strain is deficient in the DnaJ "chaperonin", and several unstable proteins have been shown to be more stable in this host. 

    Genotype

    F- thr:Tn10(TetR) dnaJ259 leu fhuA2 lacZ90(oc) lacY glnV44 thi

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • tetracycline

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. PR1031 is EcoK r+ m+, so your plasmid has to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.

    References

    1. Straus, D.B., Walter, W.A. and Gross, C.A. (1988). Genes and Devel.. 2, 1851-1858.
    2. Reidhaar-Olson, J.F., Parsell, D.A. and Sauer, R.T. (1990). Biochemistry. 29, 7563-7571.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.