E. coli K12 TB1

Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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Strain Products

Description

A suspension of E. coli strain K12 TB1 which has been grown in LB medium and brought to 50% glycerol. This strain is a derivative of JM83 that has been made hsdR so it will not restrict unmodified DNA.

Genotype

F- ara Δ(lac-proAB) [Φ80dlac Δ(lacZ)M15] rpsL(StrR) thi hsdR

Properties and Usage

Storage Temperature

-20°C

Antibiotic Resistance

  • str

Growth Media

LB

Growth Temperature

37 °C

Notes

  1. Rubidium Chloride Method: 404

References

  1. Johnston, T.C. et al. (1986). J. Biol. Chem.. 261, 4805-4811.
  2. Yanisch-Perron, C. et al. (1985). Gene. 33, 103-119.

FAQs

  1. What strain(s) do you recommend as hosts for the pMAL vectors?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.