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    E. coli K12 TB1

    Limit of 1 per order. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli strain K12 TB1 which has been grown in LB medium and brought to 50% glycerol. This strain is a derivative of JM83 that has been made hsdR so it will not restrict unmodified DNA.

    Genotype

    F- ara Δ(lac-proAB) [Φ80dlac Δ(lacZ)M15] rpsL(StrR) thi hsdR

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • str

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. Rubidium Chloride Method: 404

    References

    1. Johnston, T.C. et al. (1986). J. Biol. Chem.. 261, 4805-4811.
    2. Yanisch-Perron, C. et al. (1985). Gene. 33, 103-119.

    FAQs

    1. What strain(s) do you recommend as hosts for the pMAL vectors?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.