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  • E. coli K12 ER2267

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4103S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    A suspension of E. coli ER2267 which has been grown in rich medium and brought to 50% glycerol. MM294 background: EcoK r- m- McrA- McrBC- Mrr-.

    Genotype

    proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • kan

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. Restriction Defects: McrA, McrBC, and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (3,4).

      Restriction by each of these three restriction systems individually (tested with λ DNA modified with the Sss I methylase and then packaged into phage) is: McrA, 250-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is 5,000-fold (1). In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites (5). ER2267 is deficient in all four systems.
    2. Other Properties: Selectable F' (Kan'); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (laclq); recA stabilizes repeat sequences and reduces plasmid multimerization.
    3. Selectable F´ (KanR); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (lacIq); recA stabilizes repeat sequences and reduces plasmid multimerization.

    References

    1. Kelleher, J.E. and Raleigh, E.A. (1991). J. Bacteriol.. 173, 5220-5223.
    2. Raleigh, E.A. and Wilson, G. (1986). Proc. Natl. Acad. Sci. USA. 83, 9070-9074.
    3. Waite-Rees, P.A. et al. (1991). J. Bacteriol.. 173, 5207-5219.
    4. Heitman, J. and Model, P. (1987). J. Bacteriol.. 169, 3243-3250.
    5. Murray, N.E. et al. (1973). Mol. Gen. Genet.. 120, 261-281.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.