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  • E. coli K12 ER1821

    Limit of 1 per order
    Catalog #SizeConcentrationPriceQtyAdd to Cart
    E4102S200 μl$0.00Add to Cart
      
    Categories:
    Strain Products

    Description

    MM294 background: McrA- McrBC- EcoK r- m- Mrr- . A suspension of E. coli ER1821 which has been grown in rich medium and brought to 50% glycerol.

    Genotype

    F- glnV44 e14-(McrA-) rfbD1? relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10

    Properties and Usage

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. Restriction Defects: McrA, McrBC, and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (2).

      Mrr also restricts many methyladenine-containing sequences (3,4). Restriction by each of these three restriction systems individually (tested with λ DNA modified with the Sss I methylase and then packaged into phage) is: McrA, 250-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is 5,000-fold (1). In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites (5). ER1821 lacks all four activities.

    References

    1. Kelleher, J.E. and Raleigh, E.A. (1991). J. Bacteriol.. 173, 5220-5223.
    2. Raleigh, E.A. and Wilson, G. (1986). Proc. Natl. Acad. Sci. USA. 83, 9070-9074.
    3. Waite-Rees, P.A. et al. (1991). J. Bacteriol.. 173, 5207-5219.
    4. Heitman, J. and Model, P. (1987). J. Bacteriol.. 169, 3243-3250.
    5. Murray, N.E. et al. (1973). Mol. Gen. Genet.. 120, 261-281.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.