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  • E. coli K12 ER1793

    Limit of 1 per order. Although this product is free of charge, freight charges will apply. For US customers, available via phone ordering only (1-800-632-5227). For international customers, please contact your local NEB subsidiary or distributor.
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    Strain Products

    Description

    A suspension of E. coli ER1793 which has been grown in rich medium and brought to 50% glycerol. ER1370 background; McrA- McrBC- Mrr- EcoK r- m-

    Genotype

    F- fhuA2 Δ(lacZ)r1 glnV44 e14-(McrA-) trp-31 his-1 rpsL104 xyl-7 mtl-2 metB1 Δ(mcrC-mrr)114::IS10

    Properties and Usage

    Storage Temperature

    -20°C

    Antibiotic Resistance

    • str

    Growth Media

    LB

    Growth Temperature

    37 °C

    Notes

    1. Restriction Defects: McrA, McrBC, and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (2).
    2. Mrr also restricts many methyladenine-containing sequences (3,4). Restriction by each of these three restriction systems individually (tested with λ DNA modified with the Sss I methylase and then packaged into phage) is: McrA, 250-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is 5,000-fold (1). In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites (5). ER1793 is deficient in all four systems (1). This strain was reported to yield good quality libraries of Helicobacter pylori chromosomal DNA (6).

    References

    1. Kelleher, J.E. and Raleigh, E.A. (1991). J. Bacteriol.. 173, 5220-5223.
    2. Raleigh, E.A. and Wilson, G. (1986). Proc. Natl. Acad. Sci. USA. 83, 9070-9074.
    3. Waite-Rees, P.A. et al. (1991). J. Bacteriol.. 173, 5207-5219.
    4. Heitman, J. and Model, P. (1987). J. Bacteriol.. 169, 3243-3250.
    5. Murray, N.E. et al. (1973). Mol. Gen. Genet.. 120, 261-281.
    6. Phadnis, S.H. et al. (1993). Mol. Microbiol.. 10, 1151.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.