BioLux® Gaussia Luciferase Flex Assay Kit
- This product was discontinued on 01/05/2015
The BioLux® Gaussia Luciferase Flex Assay Kit contains reagents necessary for assaying Gaussia Luciferase activity with adjustable light emission kinetics, most commonly secreted from mammalian cells transfected with GLuc expression plasmids.
The BioLux GLuc Flex Assay Kit includes a higher concentration coelenterazine substrate and an additional stabilizer component, which allows the use of the assay in high throughput format or without the requirement of an injectorequipped luminometer. With the standard protocol the light emission decays slowly with a half-life of approximately 25 minutes. The addition of stabilizer decreases the absolute value of light output but confers signal stability over periods of time (Figure 2). This three component assay system provides the user with 2 options: (a) use the assay without stabilizer for enhanced light output or (b) use with the desired amount of stabilizer for enhanced stability.
Gaussia Luciferase is a luciferase reporter from the marine copepod Gaussia princeps (1,2). This luciferase, which does not require ATP, catalyzes the oxidation of the substrate coelenterazine in a reaction that produces light, and has considerable advantages over other luminescent reporter genes.
The luminescence measured from the supernatant of cultured cells transfected with a plasmid expressing GLuc is proportional to the amount of enzyme produced, which in turn, reflects the level of transcription. Alternatively a cell lysate can be used for the assay. Although most of the activity is secreted, the high sensitivity of GLuc allows measurements from the cellular fraction as well.
The following reagents are supplied with this product:
Store at (°C) Concentration BioLux® GLuc Flex Assay Buffer 1 X BioLux® GLuc Stabilizer -20 100 X BioLux® GLuc Flex Substrate 100 X
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