PicoPLEX™ WGA Kit
- This product was discontinued on January 01, 2017
The PicoPLEX WGA Kit, developed and manufactured by Rubicon Genomics, Inc., efficiently amplifies total DNA from single cells or their DNA equivalent about 1 million-fold to produce 2–5 micrograms of amplified DNA in under 3 hours. The kit can also be used successfully in situations where the low input amount is uncertain as it works well over a range of inputs up to thousands of cells or several ng DNA to achieve this final yield of amplified DNA. Both AT- and GC-rich regions are represented reproducibly using this kit. With single blastomere and polar bodies, the kit is able to achieve 95% amplification success rate. The kit performs reproducibly and generates greater than 90% correlation coefficients for qPCR Ct data from replicate single-cell reactions. This method features single-copy sensitivity as well as high specificity with an expected 5 PCR cycle delay between experimental samples and non-template negative controls. The PicoPLEX WGA Kit produces amplified DNA fragments suitable for Copy Number Variation (CNV) analysis using oligonucleotide aCGH or qPCR; SNP genotyping, mutation detection and sequencing.
Kit Components: #E2620S #E2620L Cell Extraction Buffer 0.1 ml 0.3 ml Extraction Enzyme Dilution Buffer 0.1 ml 0.3 ml Cell Extraction Enzyme 0.005 ml 0.015 ml Pre-Amp Reaction Mix 0.1 ml 0.275 ml Pre-Amp Enzyme 0.005 ml 0.015 ml Amplification Reaction Mix 0.35 ml 1.4 ml
Amplification Enzyme 0.012 ml 0.05 ml
Nuclease-Free Water 1.8 ml 1.8 ml
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- Isothermal Amplification & Strand Displacement
- Whole Genome Amplification
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