PicoPLEX™ WGA Kit

Features

• Single-copy specificity
• Low background
• Reproducible

Companion Products

• Gel Loading Dye, Orange (6X)
• Quick-Load® 100 bp DNA Ladder

1. Storage:
   Store the PicoPLEX WGA Kit at -20°C for up to 12 months from the assay date. The expiration date can be found on the box label.
2. Transfer the Cell Extraction Enzyme, Pre-Amp Enzyme and Amplification Enzyme tubes to ice just before use. All other components can be thawed on ice and briefly vortexed prior to use.
3. This kit is designed to amplify picogram quantities of DNA, therefore extreme caution must be exercised to prevent introduction of exogenous DNA. Good laboratory practices for PCR should be followed when storing and handling reagents as well as when setting up reactions.

1. Sample Preparation Methods for PicoPLEX WGA Kit
2. Pre-Amplification Protocol for PicoPLEX WGA Kit
3. Amplification Protocol for PicoPLEX WGA Kit

1. How many cells can be amplified by the PicoPLEX WGA Kit?
2. Does the PicoPLEX WGA Kit amplify genomic loci?
3. Are all genomic loci equally represented in products amplified from the PicoPLEX WGA Kit?
4. Does the PicoPLEX WGA Kit amplify GC-rich regions?
5. Can negative control reactions not containing a cell or DNA be distinguished from single cell reactions?
6. How robust is the WGA process?
7. How rapid is the Single Cell WGA process?
8. How long are the expected amplicons generated with the PicoPLEX WGA Kit?
9. Should cells be washed before collections?
10. Which cell collection methods are compatible with the PicoPLEX WGA Kit?
11. Are there special requirements for flow sorting?
12. What cells types have been successfully amplified by the PicoPLEX WGA Kit?
13. Can the PicoPLEX WGA Kit be used with subcellular materials?
14. Can the PicoPLEX WGA Kit be used with bacterial cells?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

FOR RESEARCH USE ONLY: Developed and manufactured by Rubicon Genomics, Inc., Ann Arbor, MI, USA. Protected under US Pat. No. 8,206,913. Other US and foreign patents pending. PICOPLEX, RUBICON GENOMICS + stylized DNA design are trademarks or registered trademarks of Rubicon Genomics, Inc., used under license.

Limited Use Label License: This product and its use is the subject of one or more issued and/or pending U.S. and foreign patents and patent applications owned by Rubicon Genomics, Inc. The purchase of this product from NEB or its authorized distributors conveys to the buyer the non-transferable right to use the purchased amount of the product for Internal Research Use Only. For use of this product for Commercial, including resale of the product or use as part of a paid-for service, or diagnostic purposes, contact NEB at [email protected]

Developed and manufactured by Rubicon Genomics, Inc.
Distributed by New England Biolabs, Inc.