As an improved alternative, NEB now offers the T7 Quick High Yield RNA Synthesis Kit. This new kit provides the same level of performance and yield as the HiScribe T7 In Vitro Transcription Kit, but with several protocol enhancements. Learn more in the Discontinuation Notice.
The HiScribe T7 In Vitro Transcription Kit is a system for the in vitro synthesis of large amounts of single-stranded (ss) or double-stranded (ds)RNA. This RNA can be used in numerous applications including RNA structural studies, ribozyme biochemistry, in vitro translation, RNA-protein interactions, antisense technology, aptamer discovery (SELEX), and RNA interference (RNAi) experiments.
The HiScribe T7 In Vitro Transcription Kit is designed for the production of RNAs that are 0.3 kb and longer (Protocol 1) or for small RNAs 50–300 nt in length (Protocol 2).
The kit contains sufficient reagents for synthesis of up to 3 mg RNA in 2 ml of total reaction volume.
DNA Template Preparation:
The sequence to be transcribed can be cloned in a plasmid downstream of a T7 promoter (e.g. LITMUS 28i or LITMUS 38i – see companion products) or generated by PCR (Figure 1).
See FAQs for Plasmid and PCR Template Preparation
The following reagents are supplied with this product:
Store at (°C) Concentration High Molecular Weight (HMW) Component Mix 20 X 10X Transcription Buffer 10 X T7 RNA Polymerase (500 units/μl) 500 units/μl StuI Linearized Litmus 28iMal Control Plasmid (1 mg/ml) 1 mg/ml Ribonucleotide Solution Mix -20 25 mM of each NTP
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