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    Synthetic Biology

    The evolution of our understanding of life sciences has opened the doors to recreate natural systems and even design biological functions and systems that do not exist in nature. NEB supports synthetic biology research by providing the NEBuilder® HiFi DNA Assembly products (NEB #E2621 and #E5520), BioBrick® Assembly Kit (NEB #E0546) and the Gibson Assembly™ Products (NEB #E2611, and #E5510). These products are helpful to the synthetic biology community because of their convenience, flexibility and suitability for a wide range of sequence sizes.

    BioBrick® is a registered trademark of the BioBricks Foundation.
    NEBuilder® is a registered trademark of New England Biolabs, Inc
    Gibson Assembly™ is a manufactured by New England Biolabs, Inc under license from synthetic Genomics, Inc
    1. Introduction to NEBuilder HiFi DNA Assembly

      Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over Gibson Assembly.

    2. Golden Gate Assembly Workflow

      Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.

    3. Introduction to Gibson Assembly

      Watch an interactive tutorial that details the process by which Gibson Assembly® joins DNA fragments in a single tube, isothermal reaction.

    4. Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly™ or Gibson Assembly™

      Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit.

    5. Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter

      See how Petar & Clinton, NEB/JoVE video abstract contest winners, have been using NEB's Gibson Assembly products in their laboratory!

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