NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.
Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Quick Dephosphorylation Kit (NEB #M0508), Shrimp Alkaline Phosphatase (NEB #M0371), Calf Intestinal Alkaline Phosphatase (NEB #M0290), and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).
ATP Sulfurylase (NEB #M0394) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate.
NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start.
- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorylation Kit (M0508)
- Enzymatic PCR Cleanup Protocol
- DNA Ligase Selection Chart
- Phosphatase Selection Chart
- Phage Display Troubleshooting Guide
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please email us at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.