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  • Phosphatases & Sulfurylases

    DNA Modifying Enzymes

    NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions or dephosphorylation of serine, threonine and tyrosine residues in proteins.

    Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Alkaline Phosphatase, Calf Intestinal (NEB #M0290) and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).

    ATP Sulfurylase (NEB #M0394) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate.

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    Common Applications of Exonucleases and Endonucleases

    NEB provides a list of common applications for our exonucleases and endonucleases.

    Properties of Exonucleases and Endonucleases

    NEB supplies many nucleases; several characteristics should be considered when choosing the one best suited to your particular research needs.