NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.
Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Quick Dephosphorylation Kit (NEB #M0508), Shrimp Alkaline Phosphatase (NEB #M0371), Calf Intestinal Alkaline Phosphatase (NEB #M0290), and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).
ATP Sulfurylase (NEB #M0394) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate.
NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start.