NEB supplies a variety of phosphatases for use in cloning, such as preparation of templates for 5´end labeling, prevention of recircularization of cloning vectors, enhancing yields of RNA in transcription reactions.
Phosphatases catalyze the removal of 5´ phosphate groups from DNA and RNA (Quick Dephosphorylation Kit (NEB #M0508), Shrimp Alkaline Phosphatase (NEB #M0371), Calf Intestinal Alkaline Phosphatase (NEB #M0290), and Antarctic Phosphatase (NEB #M0289). Inorganic phosphatases catalyze the hydrolysis of inorganic pyrophosphate to form orthophosphate (Pyrophosphatase, Inorganic (yeast) (NEB #M2403) and Thermostable Inorganic Pyrophosphatase (NEB #M0296)).
ATP Sulfurylase (NEB #M0394) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate.
NEBCloner is a guide for selecting appropriate products and viewing protocols for steps in the cloning workflow. To help select the right DNA phosphatase, choose "End Modification" on the tool to start.
Protocols for Phosphatases & Sulfurylases
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorlyation Kit (M0508)
- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Enzymatic PCR Cleanup Protocol
Application Notes for Phosphatases & Sulfurylases
Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.