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NEB offers a selection of DNA and protein methyltransferases.

DNA methyltransferases transfer a methyl group from S-adenosylmethionine to either adenine or cytosine residues and can be used to generate methylated DNA at specific sites for gene expression studies. Our selection includes CpG methyltransferase (NEB #M0226), which transfers a methyl group to the C5 position of cytosine residues and is especially useful for studying CpG methylation effects.

Several protein methyltransferases are also available for the specific methylation of lysine and arginine residues in Histone H3 and H4 for gene regulation studies.

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    Publications related to Methyltransferases
  1. Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. 2016. Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res. . Nov, PubMedID: 27580720, DOI:
  2. Ee, R., Lim, Y.L., Yin, W.F., See-Too, W.S., Roberts, R.J. and Chan, K.G. 2016. Novel Methyltransferase Recognition Motif Identified in Chania multitudinisentens RB-25(T) gen. nov., sp. nov. Front Microbiol . 206, PubMedID: 27630623 , DOI:
  3. Martinez, F.L., Anton, B.P., DasSharma, P., ​Pajal, V., Irazusta, V., Roberts, R.J., DasSharma, S. 2019. Complete genome sequence and methylome analysis of micrococcus luteus SA211, a halophilic, lithium-tolerant Actinobacterium from Argentina Microbiol Resour Announc. 8 (4), PubMedID: 30701250, DOI: 10.1128/MRA.01557-18
  4. Fomenkov, A., Vincze, T., Mersha, F., Roberts, R.J. 2018. Complete genome sequence and methylome analysis of Bacillus caldolyticus NEB414. Genome Announcements. 6 (6), PubMedID: 29439055 , DOI: 10.1128/genomeA.01605-17
  5. Pirone-Davies, C., Hoffmann, M., Roberts, R.J., Muruvanda, T., Timme, R.E., Strain, E., Luo, Y., Payne, J., Luong, K., Song, Y., Tsai, Y.C., Boitano, M., Clark, T.A., Korlach, J., Evans, P.S., Allard, M.W. 2015. Genome-Wide Methylation Patterns in Salmonella enterica Subsp. enterica Serovars PLoS One. 10(4), PubMedID: 25860355, DOI:
  6. Seib, K.L., Jen, F.E., Tan, A., Scott, A.L., Kumar, R., Power, P.M., Chen, L.T., Wu, H.J., Wang, A.H., Hill, D.M., Luyten, Y.A., Morgan, R.D., Roberts, R.J., Maiden, M.C., Boitano, M., Clark, T.A., Korlach, J., Rao, D.N. and Jennings, M.P. 2015. Specificity of the ModAll, ModA12 and ModD1 epigenetic regulator N6-adenine DNA methyltransferases of Neisseria Meningitidis Nucleic Acids Res. 43(8), PubMedID: 25845594, DOI:
  7. Fomenkov, A., Lunnen, K.D., Zhu, Z., Anton, B.P., Wilson, G.G., Vincze, T. and Roberts, R.J. 2015. Complete genome sequence and methylome analysis of bacillus strain x1 Genome Announc. 3(1), PubMedID: 25700417, DOI:
  8. Anton, B.P., Mongodin, E.F., Agrawal, S., Fomenkov, A., Byrd, D.R., Roberts, R.J. and Raleigh, E.A. 2015. Complete Genome Sequence of ER2796, a DNA Methyltransferase-Deficient Strain of Escherichia coli K-12 PLoS One. 10(5), PubMedID: 26010885, DOI:
  9. Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. 2014. REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Research, Advance Access . , PubMedID: , DOI:
  • Positive controls for methylation specific PCR or bisulfite sequencing
  • CpG-methylated gene expression studies
  • Nucleosome footprinting
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