
DNA Repair Enzymes and Structure-specific Endonucleases
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- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Control Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Standard E. coli DNA Gyrase Reaction (M0306)
- Standard Reaction Protocol for PreCR Repair Mix
- Using RecA and an oligonucleotide to form a stable triple helix
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Transfection of Cas9 HF1 into adherent cells using the Lipofectamine® RNAiMAX
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DNA Damage - the major cause of missing pieces from the DNA puzzle
- Activities of DNA Repair Enzymes and Structure-specific Endonucleases
- Properties of DNA Repair Enzymes and Structure-specific Endonucleases
- DNA Damage and PreCR
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Usage Guidelines
- Mauris, J.and Evans, T.C., Jr. (2010) A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem; 285(15), 11087-11092. PubMedID: 20129926
- Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. (2015) Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol Res; 4, 725-738. DOI: doiL 10.1039/c4tx00197d
- Gehring, A.M., Zatopek, K.M., Burkhart, B.W., Potapov, V., Santangelo, T.J., Gardner, A.F (2019) Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis DNA Repair (Amst); PubMedID: 31841800, DOI: 10.1016/j.dnarep.2019.102767
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