DNA Repair Proteins

NEB carries a selection of DNA repair enzymes for use in multiple applications:

Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
Base excision repair
DNA mismatch repair
Nucleotide excision repair
Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
Genotoxicity tests by alkaline elution or alkaline unwinding
Manipulation of DNA supercoiling using DNA Gyrase (NEB #M0306) and Topoisomerase I (NEB #M0301)
Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)

Publications related to DNA Repair Proteins:

Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. (2015). Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol. Res.. 4, 725-738. DOI: doiL 10.1039/c4tx00197d