DNA Repair Enzymes and Structure-specific Endonucleases
DNA Repair Enzymes and Structure-specific Endonucleases are enzymes which cleave DNA at a specific DNA lesion or structure. (To learn about non-specific endonucleases and exonucleases, visit here.) These enzymes can be used in a wide variety of applications such as:
- Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
- Base excision repair (BER)
- DNA mismatch repair
- Nucleotide excision repair
- Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
- Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
- Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
- Genotoxicity tests by alkaline elution or alkaline unwinding
- Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)
Helping you select the right DNA Repair Enzymes and Structure-specific Endonucleases
NEB offers a comprehensive selection of DNA repair enzymes and structure-specific endonucleases, all of which have been optimized for robust performance in streamlined workflows.
Additional tools and resources
The following resources will help you select and learn more about DNA Repair Enzymes and Structure-specific Endonucleases from NEB.
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FAQs for DNA Repair Enzymes and Structure-specific Endonucleases
- What reaction conditions were used to define Authenticase™?
- How does Authenticase™ improve the quality and fidelity of PCR gene assembly?
- How do I convert my gene of interest into oligonucleotides?
- Why do you recommend setting up two tubes for the PCR reaction containing different amounts of Authenticase™-treated samples as templates?
- Can I use Authenticase™ for genome editing applications?
- Does Authenticase™ recognize single base pair mismatches or indels (insertions/deletions)?
- What common additives inhibit Authenticase™?
- What PCR reagents are recommended for DNA amplification in genome editing (CRISPR/Cas9, TALEN, ZFN) mismatch detection assays?
- Can I use Authenticase™ genome editing (CRISPR/Cas9, TALEN, ZFN) mismatch detection assays with unpurified PCR products?
- What size PCR amplicon should I design to analyze the genomic editing efficiency?
- If my PCR reaction yield is low, can I add more than 5 µl of the PCR reaction to the digestion reaction?
- Why do I see an extra band when I run the undigested heteroduplex on a Bioanalyzer or agarose gel?
- What are the differences between Mismatch Endonuclease I (NEB #M0678) and Authenticase (NEB #M0689)?
Protocols for DNA Repair Enzymes and Structure-specific Endonucleases
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Control Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Standard E. coli DNA Gyrase Reaction (M0306)
- Standard Reaction Protocol for PreCR Repair Mix
- Using RecA and an oligonucleotide to form a stable triple helix
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Error Correction During Gene Synthesis (NEB #M0689)
- Supplemental Protocol 1: Generation of DNA fragments by PCR assembly of pooled oligos (NEB #M0689)
- Supplemental Protocol 2: Using colony PCR to identify positive clones (NEB #M0689)
- Mismatch Detection Assay (NEB #M0689)
- Transfection of EnGen® Spy Cas9 HF1 (NEB #M0667) into adherent cells using the Lipofectamine® RNAiMAX System
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DNA Damage - the major cause of missing pieces from the DNA puzzle
- Activities of DNA Repair Enzymes and Structure-specific Endonucleases
- Properties of DNA Repair Enzymes and Structure-specific Endonucleases
- DNA Damage and PreCR
Tools & Resources
Feature Articles
Selection Tools
Usage Guidelines
- Mauris, J.and Evans, T.C., Jr. (2010) A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol Chem; 285(15), 11087-11092. PubMedID: 20129926
- Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. (2015) Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol Res; 4, 725-738. DOI: doiL 10.1039/c4tx00197d
- Gehring, A.M., Zatopek, K.M., Burkhart, B.W., Potapov, V., Santangelo, T.J., Gardner, A.F (2019) Biochemical reconstitution and genetic characterization of the major oxidative damage base excision DNA repair pathway in Thermococcus kodakarensis DNA Repair (Amst); PubMedID: 31841800, DOI: 10.1016/j.dnarep.2019.102767
Publications related to DNA Repair Enzymes and Structure-specific Endonucleases
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