DNA Repair Proteins
NEB carries a selection of DNA repair enzymes for use in multiple applications:
- Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
- Base excision repair
- DNA mismatch repair
- Nucleotide excision repair
- Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
- Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
- Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
- Genotoxicity tests by alkaline elution or alkaline unwinding
- Manipulation of DNA supercoiling using DNA Gyrase (NEB #M0306) and Topoisomerase I (NEB #M0301)
- Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)
Choose Type:
Protocols for DNA Repair Proteins
- Standard E. coli DNA Gyrase Reaction (M0306)
- Using RecA and an oligonucleotide to form a stable triple helix
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Electroporation of Cas9 RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporator
- Standard Reaction Protocol for PreCR Repair Mix
- Sequential Reaction Protocol for PreCR Repair Mix
- Control Reaction Protocol for PreCR Repair Mix
- Cloning with USER Enzyme
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Determining Genome Targeting Efficiency using T7 Endonuclease I
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DNA Damage - the major cause of missing pieces from the DNA puzzle
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PCR Brochure
The PCR brochure provides product information on the wide range of DNA polymerases available from NEB, including tools for selection and troubleshooting tips.
- DNA Damage and PreCR
Other Tools & Resources
Feature Articles
Brochures
Usage Guidelines
- Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. 2015. Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol. Res.. 4, PubMedID: , DOI: doiL 10.1039/c4tx00197d
Publications related to DNA Repair Proteins
Properties of DNA Repair Enzymes
DNA Repair Glycosidases on Various Damaged Bases dsDNA
