DNA Repair Proteins

DNA Modifying Enzymes

NEB carries a selection of  DNA repair enzymes for use in multiple applications:

  • Repair of DNA sample degradation due to oxidative damage, UV radiation, ionizing radiation, phenol/chloroform extraction, mechanical shearing, formalin fixation (post extraction) or long term storage
  • Base excision repair
  • DNA mismatch repair
  • Nucleotide excision repair
  • Forensic analysis of environmental samples, analysis of ancient DNA, DNA damage control, and DNA-DNA and protein-DNA interactions
  • Preparation for downstream applications such as PCR, microarray analysis, or other DNA technologies
  • Single cell gel electrophoresis (Comet assay) to assess samples for DNA damage
  • Genotoxicity tests by alkaline elution or alkaline unwinding
  • Manipulation of DNA supercoiling using DNA Gyrase (NEB #M0306) and Topoisomerase I (NEB #M0301)
  • Elimination or repair of large DNA secondary structures using T7 Endonuclease I (NEB #M0302)

    Publications related to DNA Repair Proteins:

  1. Karbaschi, Mahsa., Macip, Salvador., Mistry, Vilas., Abbas, Hussein H.K., Delinassios, George J., Evans, Mark D., Young, Antony R., Cooke, Marcus S. (2015). Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response Toxicol. Res.. 4, 725-738. DOI: doiL 10.1039/c4tx00197d

Properties of DNA Repair Enzymes

DNA Repair Glycosidases on Various Damaged Bases dsDNA