The list of known prokaryotic argonautes is continually growing as the field expands. Some examples of DNA:DNA argonautes (meaning DNA-guided DNA-endonuclease) include those from Pyrococcus furiosus (PfAgo), Thermus thermophilus (TtAgo), and Methanocaldococcus jannaschii (MjAgo). RNA:DNA argonautes include those from Marinitoga piezophila (MpAgo), Thermotoga profunda (TpAgo), and Rhodobacter sphaeroides (RsAgo). DNA:RNA argonautes include those from Aquifex aeolicus (AaAgo) and (with reduced activity) Thermus thermophilus (TtAgo). Finally, RNA:RNA argonautes include those from Marinitoga piezophila (MpAgo), and for comparison, eukaryotic argonaute human (AGO2).
NEB now offers the first commercially available argonaute Tth Argonaute (TtAgo) (NEB #M0665), a DNA-guided DNA-endonuclease for in vitro applications.
- GUIDElines for optimization of Tth Argonaute (TtAgo) reactions
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Learn about prokaryotic argonautes, which are nucleic acid-guided endonucleases that can be used as programmable nucleases. TtAgo is the first commercially available prokaryotic argonaute.