Discontinued (<3 years)
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Protocols for Discontinued (<3 years)
- 2: In Vitro Transcription of Short Templates (50-300 nt) (E2030)
- 5 Minute Transformation Protocol (C3009)
- 5 Minute Transformation Protocol (C3016)
- Adaptor Ligation of End Repaired DNA (E6260)
- Adaptor or Vector Ligation of cDNA Library (E6114)
- Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
- Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- Agencourt Ampure Beads (Beckman Coulter) Preparation (E6116)
- Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
- Cellular Labeling (S9126)
- Cellular Labeling (S9131)
- Cellular Labeling (S9137)
- Cellular Labeling (S9139)
- Cellular Labeling (S9142)
- Cellular Labeling (S9216)
- Cellular Labeling (S9218)
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Cloning with USER Enzyme
- CLuc Activity Assay Protocol II (Injector-equipped luminometers) (E3314)
- CLuc Activity Assay Protocol I (Luminometers without injectors) (E3314)
- Combination of TransPass D1 or TransPass D2 & TransPass V (M2561)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- PCR Protocol for Crimson Taq DNA Polymerase (M0324)
- PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
- Detection by Fluorescent Imager (P9311)
- Detection using the Phototope-Star Detection Kit
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of MCP Fusions (N9317)
- Expression of MCP-GPI (N9320)
- First Strand cDNA Synthesis (E6114)
- First Strand cDNA Synthesis
- High Efficiency Transformation Protocol (C3009)
- High Efficiency Transformation Protocol (C3016)
- Cellular Labeling (S9101)
- Instructions for Cellular Labeling (S9215)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9222)
- Labeling CLIP-tag Purified Protein In Vitro (P9311)
- Labeling Mammalian Cell Lysates (S9146)
- Labeling Mammalian Cell Lysates (S9235)
- Labeling of Proteins in vitro (S9137)
- Labeling of Proteins in vitro (S9216)
- Labeling of Proteins in vitro (S9218)
- Labeling of Proteins in vitro (S9101)
- Labeling Proteins in vitro (S9131)
- Labeling proteins in vitro (S9126)
- Labeling Proteins in vitro (S9139)
- Labeling Proteins in vitro (S9142)
- Labeling Proteins in vitro (S9146)
- Labeling Purified Proteins in vitro (S9235)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9149)
- Ligation of 3´ and 5´ Adaptors (E6120)
- Loading a Sample (P7708)
- M13 Amplifcation Protocol for Ph.D. Phage Display
- miRNA Detection (E3312)
- mRNA Fragmentation Protocol (E6114)
- mRNA Fragmentation Protocol (E6116)
- NEBNext Adaptor Ligation (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
- NEBNext Adaptor Ligation (E6116)
- NEBNext End Repair and dA-Tailing (E6116)
- NEBNext End Repair and dA-Tailing (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- NEBNext End Repair Module Protocol (E6060)
- NEBNext End Repair Module Protocol (E6260)
- NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
- NEBNext Quick Ligation Module Protocol (E6060)
- NEBNext Small Fragment Removal (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
- NEBNext Small Fragment Removal (E6116)
- Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
- PCR Amplification (E6120)
- Perform a Second Purification of the Amplified DNA (E6260)
- Preparing and Loading Protein Ladders (P7711)
- Protocol for a Typical miRNA Methylation Reaction (M0228)
- Protocol for Expression Using T7 Express Sampler (C3009)
- Protocol for Expression Using T7 Express Iq (C3016)
- Protocol II (Injector-equipped luminometers) (E3308)
- Protocol I (Luminometers without injectors) (E3308)
- Purification of Adaptor Ligated DNA (E6260)
- Purification of Amplified DNA (E6260)
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Reconstitution of BioLux Cypridina Luciferase Substrate (E3314)
- Reverse Transcription (E6120)
- RNase Contamination Assay Kit (E3320)
- Second Strand cDNA Synthesis (E6114)
- ShortCut® siRNA Mix Suggested Protocol
- Suggested Protocol for Loading a Sample (P7702)
- Suggested Protocol for Loading a Sample (P7709)
- PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
- Transfection Guidelines (E3314)
- Transformation Protocol (C1007)
- Transformation K. lactis YCT284 Competent Cells (C1002)
- TransPass COS/293: Transfection Protocol
- TransPass D1 Protocol 1: Transfection in the presence of serum
- TransPass D1 Protocol 2: Transfection in the absence of serum
- TransPass D2 Protocol 1: Transfection in the presence of serum
- TransPass D2 Protocol 2: Transfection in the absence of serum
- TransPass™ HUVEC Transfection Reagent: Transfection Protocol
- TransPass P Protein: Transfection Protocol
- TransPass R2: Plasmid DNA and siRNA Transfection Protocol
- TransPass R2: siRNA Transfection Protocol
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Western Transfer Protocol for Anti-MBP Antiserum
- NEBNext® RNase III RNA Fragmentation Module (E6146)
- Pmal Piii Vector Protocol
- Reaction Conditions for Chemical Coupling with CoA-SH (S9352S)
- Labeling of Proteins in vitro (S9215)
- Control Reactions with ProtoScript® M-MuLV Taq RT-PCR Kit
- Standard Digest Using RE-Mix®
- Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
- Expression of SNAPf Fusions (N9185)
- Expression of SNAPf Fusions in SNAPf-H2B (N9186)
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Amplification Protocol for PicoPLEX WGA Kit
- First Strand cDNA Synthesis with AMV LongAmp™ Taq RT-PCR Kit
- First Strand cDNA Synthesis with ProtoScript® M-MuLV Taq RT-PCR Kit
- PCR Amplification with ProtoScript® M-MuLV Taq RT-PCR Kit
- Pre-Amplification Protocol for PicoPLEX WGA Kit
- Sample Preparation Methods for PicoPLEX WGA Kit
- Double Digest Protocol using Two RE-Mix® Enzymes
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9155)
- Protocol for Labeling ACP- or MCP-tag Fusion Proteins with CoA Substrates.
- Reaction Conditions for Chemical Coupling (S9155)
- Reaction Conditions for Chemical Coupling (S9237)
- LongAmp™ Taq 2X Master Mix Protocol (E6060)
- NEBNext End Repair cDNA Library (E6114)
- PCR Amplification of Adaptor Ligated cDNA Library (E6114)
- PCR Protocol for Crimson LongAmp™ Taq DNA Polymerase (M0326)
- Expression of CLIPf-NK1R (N9216)
- Expression of CLIPf-Cox8A (N9217)
- Expression of CLIPf-H2B (N9218)
- Typical Reaction Conditions (P0732)
- Reaction Conditions for Chemical Coupling (S9222)
- Reaction Conditions for Chemical Immobilization (S9149)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)
- M13 Titer Protocol for Ph.D. Phage Display
- Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- Please see manual (NEB #E6000) for protocols.
- Please see manual (NEB #E6100) for protocols
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
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