Discontinued
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Protocols for Discontinued
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
- Please see manual (NEB #E6100) for protocols
- Please see manual (NEB #E6000) for protocols.
- ShortCut® siRNA Mix Suggested Protocol
- High Efficiency Transformation Protocol (C3016)
- Preparing and Loading Protein Ladders (P7711)
- PCR Amplification (E6120)
- Perform a Second Purification of the Amplified DNA (E6260)
- Protocol for Expression Using T7 Express Iq (C3016)
- Protocol for Expression Using T7 Express Sampler (C3009)
- Protocol for a Typical miRNA Methylation Reaction (M0228)
- mRNA Fragmentation Protocol (E6114)
- mRNA Fragmentation Protocol (E6116)
- miRNA Detection (E3312)
- Ligation of 3´ and 5´ Adaptors (E6120)
- Labeling Proteins in vitro (S9146)
- Labeling Proteins in vitro (S9142)
- Labeling Purified Proteins in vitro (S9235)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9149)
- NEBNext Small Fragment Removal (E6080)
- NEBNext Quick Ligation Module Protocol (E6060)
- NEBNext Small Fragment Removal (E6116)
- Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
- NEBNext Adaptor Ligation (E6116)
- NEBNext End Repair and dA-Tailing (E6116)
- NEBNext Adaptor Ligation (E6080)
- NEBNext End Repair and dA-Tailing (E6080)
- NEBNext End Repair Module Protocol (E6260)
- NEBNext End Repair Module Protocol (E6060)
- Transformation K. lactis YCT284 Competent Cells (C1002)
- Transformation Protocol (C1007)
- Suggested Protocol for Loading a Sample (P7709)
- Suggested Protocol for Loading a Sample (P7702)
- Transfection Guidelines (E3314)
- Western Transfer Protocol for Anti-MBP Antiserum
- TransPass P Protein: Transfection Protocol
- TransPass R2: Plasmid DNA and siRNA Transfection Protocol
- TransPass R2: siRNA Transfection Protocol
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Reconstitution of BioLux Cypridina Luciferase Substrate (E3314)
- Protocol I (Luminometers without injectors) (E3308)
- Purification of Adaptor Ligated DNA (E6260)
- Purification of Amplified DNA (E6260)
- Protocol II (Injector-equipped luminometers) (E3308)
- RNase Contamination Assay Kit (E3320)
- Reverse Transcription (E6120)
- Second Strand cDNA Synthesis (E6114)
- Second Strand cDNA Synthesis
- Cellular Labeling (S9218)
- Cellular Labeling (S9142)
- Cellular Labeling (S9216)
- Cellular Labeling (S9139)
- Cellular Labeling (S9126)
- Cellular Labeling (S9137)
- Cellular Labeling (S9131)
- Detection by Fluorescent Imager (P9311)
- Detection using the Phototope-Star Detection Kit
- Cloning with USER Enzyme
- CLuc Activity Assay Protocol I (Luminometers without injectors) (E3314)
- CLuc Activity Assay Protocol II (Injector-equipped luminometers) (E3314)
- 5 Minute Transformation Protocol (C3009)
- 5 Minute Transformation Protocol (C3016)
- 1: In Vitro Transcription of Long Templates (> 0.3 kb) (E2030)
- 2: In Vitro Transcription of Short Templates (50-300 nt) (E2030)
- Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
- Adaptor Ligation of End Repaired DNA (E6260)
- Adaptor or Vector Ligation of cDNA Library (E6114)
- Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
- Agencourt Ampure Beads (Beckman Coulter) Preparation (E6116)
- Labeling Mammalian Cell Lysates (S9146)
- Labeling Mammalian Cell Lysates (S9235)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9222)
- Labeling CLIP-tag Purified Protein In Vitro (P9311)
- Instructions for Cellular Labeling (S9215)
- Labeling Proteins in vitro (S9139)
- Labeling proteins in vitro (S9126)
- Labeling Proteins in vitro (S9131)
- Labeling of Proteins in vitro (S9216)
- Labeling of Proteins in vitro (S9218)
- Labeling of Proteins in vitro (S9137)
- High Efficiency Transformation Protocol (C3009)
- First Strand cDNA Synthesis (E6114)
- First Strand cDNA Synthesis Protocols (E6550)
- First Strand cDNA Synthesis
- Control Reactions with ProtoScript® M-MuLV Taq RT-PCR Kit
- Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
- Reaction Conditions for Chemical Coupling with CoA-SH (S9352S)
- Labeling of Proteins in vitro (S9215)
- PCR Protocol for Crimson LongAmp™ Taq DNA Polymerase (M0326)
- PCR Amplification with ProtoScript® M-MuLV Taq RT-PCR Kit
- PCR Amplification with AMV LongAmp™ Taq RT-PCR Kit
- Sample Preparation Methods for PicoPLEX WGA Kit
- Pre-Amplification Protocol for PicoPLEX WGA Kit
- Amplification Protocol for PicoPLEX WGA Kit
- First Strand cDNA Synthesis with AMV LongAmp™ Taq RT-PCR Kit
- First Strand cDNA Synthesis with ProtoScript® M-MuLV Taq RT-PCR Kit
- NEBNext End Repair cDNA Library (E6114)
- PCR Amplification of Adaptor Ligated cDNA Library (E6114)
- LongAmp™ Taq 2X Master Mix Protocol (E6060)
- Protocol for Labeling ACP- or MCP-tag Fusion Proteins with CoA Substrates.
- Reaction Conditions for Chemical Coupling (S9237)
- Reaction Conditions for Chemical Coupling (S9155)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9155)
- Cell Surface Labeling ACP-Surface Starter Kit
- Labeling of Proteins in vitro for ACP-Surface Starter Kit
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Expression of SNAPf Fusions in SNAPf-H2B (N9186)
- Expression of SNAPf Fusions (N9185)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- Reaction Conditions for Chemical Immobilization (S9149)
- Reaction Conditions for Chemical Coupling (S9222)
- Typical Reaction Conditions (P0732)
- NEBNext® RNase III RNA Fragmentation Module (E6146)
- Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)
- M13 Titer Protocol
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
- Loading a Sample (P7708)
- M13 Amplification
- TransPass D2 Protocol 1: Transfection in the presence of serum
- TransPass D2 Protocol 2: Transfection in the absence of serum
- TransPass™ HUVEC Transfection Reagent: Transfection Protocol
- TransPass COS/293: Transfection Protocol
- TransPass D1 Protocol 1: Transfection in the presence of serum
- TransPass D1 Protocol 2: Transfection in the absence of serum
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Combination of TransPass D1 or TransPass D2 & TransPass V (M2561)
- Cellular Labeling (S9101)
- Labeling of Proteins in vitro (S9101)
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of MCP-GPI (N9320)
- Standard Digest Using RE-Mix®
- Pmal Piii Vector Protocol
- Expression of CLIPf-NK1R (N9216)
- Expression of CLIPf-H2B (N9218)
- Expression of CLIPf-Cox8A (N9217)
- Double Digest Protocol using Two RE-Mix® Enzymes
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
- PCR Protocol for Crimson Taq DNA Polymerase (M0324)
- PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- Expression of MCP Fusions (N9317)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
