Competent Cells

Cloning Strains

Ensure successful transformations with NEB competent E. coli for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value.

NEB Competent E. coli – building a strong foundation for your cloning experiments.

  1. How to Perform a Transformation with NEB Competent Cells

    Use this protocol for maximum transformation efficiency.

  2. Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.

Advantages of NEB Competent Cells

  • Free of animal products 
  • T1 phage resistance (fhuA2) 
  • Media and control plasmid included with most strains 
  • A variety of convenient formats, including single use transformation tubes and, on request, 96 well plates 
  • Bulk sales capabilities with custom packaging

Advantages of NEB Cloning Strains

  • High transformation efficiencies 
  • EndA deficient for high quality plasmid preparation 
  • T1 phage resistant 
  • RecA deficient for reduced recombination of cloned DNA 
  • Free of animal products 
  • Suitable for blue/white screening 
  • Available as single-use tubes or multiple use aliquots 
  • Outgrowth media and control plasmid included 
  • Bulk sales capabilities with custom packaging formats

Save on Shipping Charges

 
Save money on shipping charges when purchasing competent cells from NEB

NEB Competent E. coli Strains

NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.

High Transformation Efficiencies with NEB Competent E. coli

Benefit from high transformation efficiencies with NEB Competent E. coli.