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Cloning Strains

Ensure successful transformations with NEB competent E. coli for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value.

  • High transformation efficiencies
  • Compatible with NEBuilder® HiFi DNA Assembly and Gibson Assembly® reactions, as well as ligation reactions
  • Strains also available for cloning of toxic genes
  • All strains are free of animal products and are T1 phage resistant
  • Outgrowth medium and control plasmid are included
  • Choose from a variety of convenient formats, including single use tubes
  • Bulk formats and custom packaging are available
  • Take advantage of value pricing and no dry ice charges

To help select the best strain for your experiment, please use NEBcloner®, our Cloning Competent Cell Selection Chart or our Strain Properties chart.


FAQs for Cloning Strains

Protocols for Cloning Strains

Advantages of NEB Competent Cells

  • Free of animal products 
  • T1 phage resistance (fhuA2) 
  • Media and control plasmid included with most strains 
  • A variety of convenient formats, including single use transformation tubes and, on request, 96 well plates 
  • Bulk sales capabilities with custom packaging

Advantages of NEB Cloning Strains

  • High transformation efficiencies 
  • EndA deficient for high quality plasmid preparation 
  • T1 phage resistant 
  • RecA deficient for reduced recombination of cloned DNA 
  • Free of animal products 
  • Suitable for blue/white screening 
  • Available as single-use tubes or multiple use aliquots 
  • Outgrowth media and control plasmid included 
  • Bulk sales capabilities with custom packaging formats

Save on Shipping Charges

Save money on shipping charges when purchasing competent cells from NEB

NEB Competent E. coli Strains

NEB offers several stains with varying levels of expression control, each with phage T1 resistance and extremely high transformation efficiencies.

High Transformation Efficiencies with NEB Competent E. coli

Transformation efficiencies were compared using manufacturers’ recommended protocols. Values shown are the average of triplicate experiments.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. TransformationProtocol_VideoThumb_282x210_0616

    How to Perform a Transformation with NEB Competent Cells

    Use this protocol for maximum transformation efficiency.

  2. TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.