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Cloning Competent Cell Strains

Ensure successful transformations with NEB competent E. coli for cloning. Choose from several high efficiency competent cell strains in a variety of formats that deliver performance, convenience and value.

Advantages:
  • High transformation efficiencies
  • Compatible with NEBuilder® HiFi DNA Assembly and Gibson Assembly® reactions, as well as ligation reactions. No dilution required!
  • Strains also available for cloning of toxic genes
  • All strains are free of animal products and are T1 phage resistant
  • Outgrowth medium and control plasmid are included
  • Choose from a variety of convenient formats, including single use tubes
  • Bulk formats and custom packaging are available
  • Take advantage of value pricing and no dry ice charges

Competent Cell Selection Guide

Product NEB # Characteristics Efficiency
NEB® 5-alpha Competent E. coli (High Efficiency) C2987U
C2987R
C2987P
C2987I
C2987H
  • Versatile cloning strain
  • DH5α™ derivative
1 - 3 x 109 cfu/μg,
For C2987R: 1 - 5 x 108 cfu/μg
NEB® 10-beta Competent E. coli (High Efficiency) C3019I
C3019H
  • Large plasmid and BAC cloning
  • DH10B™ derivative
1-3 x 109 cfu/µg
NEB® Stable Competent E. coli (High Efficiency) C3040I
C3040H
  • High quality plasmid preparations due to endA mutation
  • Rapid growth recA strain
  • T1 phage resistance (fhuA)
  • Value pricing
  • Free of animal products
1-3 x 109 cfu/µg
NEB® Turbo Competent E. coli (High Efficiency) C2984I
C2984H
  • Fastest growth – colonies visible after 6.5 hours
  • Plasmid preparation after 4 hours
  • Tight control/expression of toxic genes (F´lacIq)
1-3 x 109 cfu/µg
dam-/dcm- Competent E. coli C2925I
C2925H
  • Dam/Dcm methyltransferase free plasmid growth
1-3 x 106 cfu/µg
NEB® 5-alpha F'Iq Competent E. coli (High Efficiency) C2992I
C2992H
  • Toxic gene cloning
  • F´ strain with extremely high transformation efficiency
1-3 x 109 cfu/µg

 

NEB Competent E. coli Sampler

Try all four cloning strains in our NEB Competent E. coli Sampler

NEB_TE_Comp_Expressions_0617

To help select the best strain for your experiment, please use NEBcloner®, our Cloning Competent Cell Selection Chart or our Strain Properties chart.

Competent Cell Formats

Formats NEB # Single-use 200 µl tubes 96-well plate Electrocompetent Subcloning Outgrowth Medium & Control Plasmid Included
NEB 5-alpha C2987
NEB 10-beta C3019  
NEB Stable C3040  

NEB Turbo C2984  
dam-/dcm- C2925  

NEB 5-alpha F'Iq C2992    

Choose Type:

FAQs for Cloning Competent Cell Strains

Protocols for Cloning Competent Cell Strains

Advantages of NEB Competent Cells

  • Free of animal products 
  • T1 phage resistance (fhuA2) 
  • Media and control plasmid included with most strains 
  • A variety of convenient formats, including single use transformation tubes and, on request, 96 well plates 
  • Bulk sales capabilities with custom packaging

Advantages of NEB Cloning Strains

  • High transformation efficiencies 
  • EndA deficient for high quality plasmid preparation 
  • T1 phage resistant 
  • RecA deficient for reduced recombination of cloned DNA 
  • Free of animal products 
  • Suitable for blue/white screening 
  • Available as single-use tubes or multiple use aliquots 
  • Outgrowth media and control plasmid included 
  • Bulk sales capabilities with custom packaging formats

High Transformation Efficiencies with NEB Competent E. coli



NEB_TE_Comp_Expressions_0617
Transformation efficiencies were compared using manufacturers’ recommended protocols. Values shown are the average of triplicate experiments.