To enable researchers to quickly and easily begin using our protein labeling system, our Starter Kits include all the components necessary to covalently attach either a red or a green fluorophore to SNAP-tag®, CLIP-tag™ or ACP-tag fusion proteins in living cells, fixed cells or in vitro. Each Starter Kit contains a plasmid encoding the tag of interest and two cell permeable or non-cell permeable fluorescent labels. A positive control plasmid encoding a tagged protein with a well-characterized sub-cellular localization is also included (e.g., membrane, nucleus, etc.). Where available, a negative control blocking agent is included that interacts with the tag of interest, but is non-fluorescent.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, Inc.
FAQs for Starter Kits
Protocols for Starter Kits
- Labeling of Proteins in vitro (E9100)
- Cellular Labeling (E9120)
- Labeling Proteins in vitro (E9200)
- Labeling Proteins in vitro (E9120)
- Instructions for Cellular Labeling (E9200)
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100)
- Cellular Labeling (E9100)
- Cellular Labeling (E9230)
- Labeling of Proteins in Solution (E9230)
Cellular Imaging and Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
SNAP-tag® Technologies: Novel Tools to Study Protein Function
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
SNAP-Cell Starter Kit
SNAP-Suface Starter Kit
CLIP-Cell Starter Kit
CLIP-Surface Starter Kit
ACP MCP-Surface Starter Kit
Starter Kit Selection Chart
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.