To enable researchers to quickly and easily begin using our protein labeling system, our Starter Kits include all the components necessary to covalently attach either a red or a green fluorophore to SNAP-tag®, CLIP-tag™ or ACP-tag fusion proteins in living cells, fixed cells or in vitro. Each Starter Kit contains a plasmid encoding the tag of interest and two cell permeable or non-cell permeable fluorescent labels. A positive control plasmid encoding a tagged protein with a well-characterized sub-cellular localization is also included (e.g., membrane, nucleus, etc.). Where available, a negative control blocking agent is included that interacts with the tag of interest, but is non-fluorescent.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, Inc.
FAQs for Starter Kits
Protocols for Starter Kits
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Use of SNAP-Cell Block with SNAP-Cell Substrates (E9100)
- Cellular Labeling (E9100)
- Cellular Labeling (E9230)
- Labeling of Proteins in Solution (E9230)
- Labeling Proteins in vitro (E9200)
- Labeling Proteins in vitro (E9120)
- Instructions for Cellular Labeling (E9200)
- Labeling of Proteins in vitro (E9100)
- Cellular Labeling (E9120)
SNAP-tag® Technologies: Novel Tools to Study Protein Function
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
SNAP-Cell Starter Kit
SNAP-Suface Starter Kit
CLIP-Cell Starter Kit
CLIP-Surface Starter Kit
ACP MCP-Surface Starter Kit
Starter Kit Selection Chart
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.