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Starter Kits

To enable researchers to quickly and easily begin using our protein labeling system, our Starter Kits include all the components necessary to covalently attach either a red or a green fluorophore to SNAP-tag®, CLIP-tag™ or ACP-tag fusion proteins in living cells, fixed cells or in vitro. Each Starter Kit contains a plasmid encoding the tag of interest and two cell permeable or non-cell permeable fluorescent labels. A positive control plasmid encoding a tagged protein with a well-characterized sub-cellular localization is also included (e.g., membrane, nucleus, etc.). Where available, a negative control blocking agent is included that interacts with the tag of interest, but is non-fluorescent.

SNAP-tag® is a registered trademark of New England Biolabs, Inc.
CLIP-tag™ is a trademark of New England Biolabs, Inc.

FAQs for Starter Kits

Protocols for Starter Kits

SNAP-Cell Starter Kit

  • Cell-permeable
  • Labels spread across the visible spectrum, ranging from blue to red 
  • Non-fluorescent cell-permeable blocking agent available

SNAP-Suface Starter Kit

  • Non-cell permeable
  • Label SNAP-tag fusion proteins on the surface of living cells, in fixed cells or in vitro
  • Labels spread across the visible and near-infrared spectrum including photostable AlexaFluor® dyes
  • Non-fluorescent blocking agent available
  • Use SNAP-Vista Blue or Green to visualize proteins directly in SDS-PAGE

CLIP-Cell Starter Kit

  • Cell-permeable
  • Label CLIP-tag fusion proteins inside living cells, on cell surfaces or in vitro
  • Simultaneous labeling of 2 expressed proteins when used with SNAP-tag
  • Labels spread across the visible spectrum, ranging from blue to red 
  • Non-fluorescent cell-permeable blocking agent available

CLIP-Surface Starter Kit

  • Non-cell permeable
  • Label CLIP-tag fusion proteins on the surface of living cells, in fixed cells or in vitro
  • Simultaneous labeling of 2 expressed proteins when used with SNAP-tag
  • Labels include fluorophores at commonly used areas of the visible spectrum (488, 547 and 647nm)

ACP MCP-Surface Starter Kit

Require a specific synthase to label their protein target using ACP Synthase or SFP Synthase

Starter Kit Selection Chart

NEB's Starter Kits include all the components necessary to covalently attach either a red or a green fluorophore to SNAP-tag®, CLIP-tag™ or ACP-tag fusion proteins in living cells, fixed cells or in vitro.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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    Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

    Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

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    SNAP-tag Overview Tutorial

    View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.