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SNAP-tag® Substrates

NEB offers a large selection of fluorescent labels (substrates) for SNAP-fusion proteins. SNAP-tag® substrates consist of a fluorophore conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. Substrates label the SNAP-tag® without the need for additional enzymes. Cell-permeable substrates (SNAP-Cell®) are suitable for both intracellular and cell-surface labeling, whereas non-cell-permeable substrates (SNAP-Surface®) are specific for fusion proteins expressed on the cell surface only.

SNAP-tag®, SNAP-Cell® and SNAP-Surface® are registered trademarks of New England Biolabs, Inc.


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  4. Meyer B.H. et al. 2006. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells Proc. Natl. Acad. Sci. USA . 103, PubMedID: 16461466, DOI:
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  8. Yin J. et al. 2005. Labeling proteins with small molecules by site-specific posttranslational modification J Am Chem Soc. 126 , PubMedID: 15212504, DOI:
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  21. La Clair, J.J. et al. 2004. Manipulation of carrier proteins in antibiotic biosynthesis Chem. Biol. . 11, PubMedID: 15123281, DOI:
  22. George N. et al. 2004. Specific labeling of cell surface proteins with chemically diverse compounds J .Am. Chem. Soc.  . 126, PubMedID: 15264811, DOI: