Vectors are available for SNAP-, CLIP-, ACP- and MCP-tag fusion protein expression and labeling in mammalian and bacterial systems. The mammalian SNAPf and CLIPf vectors express faster-reacting variants of the SNAP- and CLIP-tags than previously available vectors. Improved polylinker sequences both upstream and downstream from the tag allow expression of the tag on either end of the protein of interest, under control of the CMV promoter. SNAPf-tag and CLIPf-tag expression vectors contain a neomycin resistance (NeoR) gene for selection of stable transfectants, together with an IRES element for efficient expression of both the fusion protein and NeoR. The ACP-tag and MCP-tag expression vectors are under control of the CMV promoter. The ACP-tag expression vector (NEB #N9322) is suitable for transient or stable expression but the MCP-tag expression (NEB #N9317) vector is suitable for transient expression only. Codon usage has been optimized for mammalian expression. Control plasmids encoding fusion proteins that are localized to the nucleus [(H2B) (NEB #N9186)], mitochondria [(Cox8A) (NEB #N9185)] and cell surface [ADRβ2 (NEB #N9184), NK1R (NEB #N9216), GPI (NEB #N9320)] are also available.The pSNAP-tag®(T7)-2 Vector (NEB #N9181) is an E.coli expression plasmid encoding the SNAP-tag protein. The codon usage of the SNAP26b gene is optimized for expression in E. coli. Expression is under control of the IPTG-inducible T7 promoter.
FAQs for Cloning Vectors & Control Plasmids
Protocols for Cloning Vectors & Control Plasmids
- Expression of SNAPf Fusions (N9183)
- Cloning of SNAP-tag Fusions in pSNAPf (N9183)
- Cloning of SNAP-tag Fusions in pSNAP-tag(T7)-2 (N9181)
- Expression of SNAP-tag Fusions (N9181)
- Cloning of CLIP-tag Fusions in pCLIPf (N9215)
- Expression of CLIP-tag Fusions (N9215)
- Double Digest Protocol with Standard Restriction Enzymes
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
- Optimizing Restriction Endonuclease Reactions
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
SNAP-tag® Technologies: Novel Tools to Study Protein Function
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
SNAP-tag®/CLIP-tag® Cloning Vector and Plasmid Selection Chart
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.