For advanced users with novel probes interested in working with SNAP-tag® technologies, building blocks are available for linkage of the core benzylguanine (BG) and benzylcytosine (BC) moieties to activated esters, primary amines and thiol groups. The variety of functional groups allows a choice of chemical coupling approaches to suit the molecule or surface to be coupled. Couple onto surfaces such as Biacore® chips or microarrays for specific protein immobilization. Couple onto peptides, proteins and DNA oligomers. Couple onto new fluorophores or affinity reagents for specific protein labeling. Labeling is gentle, precise, and versatile: one label is covalently bound under biological conditions in a defined position.
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Expression of MCP Fusions (N9317)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9150)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
- Reaction Conditions for Chemical Coupling (S9148)
- Reaction Conditions for Chemical Coupling (S9153)
- Reaction Conditions for Chemical coupling (S9150)
- Reaction Conditions for Chemical Coupling (S9151)
- Reaction Conditions for Chemical Coupling (S9236)
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
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Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.