Cellular Analysis

Building Blocks

For advanced users with novel probes interested in working with SNAP-tag® technologies, building blocks are available for linkage of the core benzylguanine (BG) and benzylcytosine (BC) and coenzyme A (CoA) moieties to activated esters, primary amines and thiol groups. The variety of functional groups allows a choice of chemical coupling approaches to suit the molecule or surface to be coupled. Couple onto surfaces such as Biacore® chips or microarrays for specific protein immobilization. Couple onto peptides, proteins and DNA oligomers. Couple onto new fluorophores or affinity reagents for specific protein labeling. Labeling is gentle, precise, and versatile: one label is covalently bound under biological conditions in a defined position.

SNAP-tag® is a registered trademark of England Biolabs, Inc.
Biacore® is a registered trademark of Biacore Life Sciences, Inc.

Videos

  1. Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

    Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

  2.

    SNAP-tag Overview Tutorial

    View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.

Building Block Selection Chart

NEB offers a variety of building blocks for advanced users with novel probes interested in working with SNAP-tag technologies. Building blocks are available for linkage of the core benzylguanine (BG) and benzylcytosine (BC) and CoA moieties to activated esters, primary amines and thiol groups.