For advanced users with novel probes interested in working with SNAP-tag® technologies, building blocks are available for linkage of the core benzylguanine (BG) and benzylcytosine (BC) and coenzyme A (CoA) moieties to activated esters, primary amines and thiol groups. The variety of functional groups allows a choice of chemical coupling approaches to suit the molecule or surface to be coupled. Couple onto surfaces such as Biacore® chips or microarrays for specific protein immobilization. Couple onto peptides, proteins and DNA oligomers. Couple onto new fluorophores or affinity reagents for specific protein labeling. Labeling is gentle, precise, and versatile: one label is covalently bound under biological conditions in a defined position.
SNAP-tag® is a registered trademark of England Biolabs, Inc.
Biacore® is a registered trademark of Biacore Life Sciences, Inc.
FAQs for Building Blocks
Protocols for Building Blocks
- Reaction Conditions for Chemical Coupling (S9153)
- Reaction Conditions for Chemical Coupling (S9148)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9148)
- Reaction Conditions for Chemical Coupling (S9151)
- Reaction Conditions for Chemical Coupling (S9236)
- Reaction Conditions for Chemical coupling (S9150)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9151)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9150)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9236)
- Expression of MCP Fusions (N9317)
SNAP-tag® Technologies: Novel Tools to Study Protein Function
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
Building Block Selection Chart
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.