For advanced users with novel probes interested in working with SNAP-tag® technologies, building blocks are available for linkage of the core benzylguanine (BG) and benzylcytosine (BC) and coenzyme A (CoA) moieties to activated esters, primary amines and thiol groups. The variety of functional groups allows a choice of chemical coupling approaches to suit the molecule or surface to be coupled. Couple onto surfaces such as Biacore® chips or microarrays for specific protein immobilization. Couple onto peptides, proteins and DNA oligomers. Couple onto new fluorophores or affinity reagents for specific protein labeling. Labeling is gentle, precise, and versatile: one label is covalently bound under biological conditions in a defined position.
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