Blocking Agents

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Blocking agents are non-fluorescent compounds that block the reactivity of the SNAP- or CLIP-tag intracellularly (SNAP-Cell® Block, NEB #S9106; and CLIP-Cell™ Block, NEB #S9220) or on the surface of live cells (SNAP-Surface® Block, NEB #S9143; and CLIP-Cell Block). They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or CLIP-tag fusion proteins. 

SNAP- and CLIP-Cell Block are highly membrane permeable and once in the cell react with the SNAP- or CLIP-tag, irreversibly inactivating it for subsequent labeling steps. 

SNAP-Surface Block also reacts with the SNAP-tag irreversibly, inactivating it for subsequent labeling steps. This blocker is largely membrane impermeable essentially limiting blocking to cell surface-exposed SNAP-tags. CLIP-Cell Block may also be used to inactivate cell supface exposed CLIP-tags.

SNAP-Cell® and SNAP-Surface® are registered trademarks of New England Biolabs, Inc.
CLIP-Cell is a trademark of New England Biolabs, Inc.

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Protocols for Blocking Agents

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    Brochures

  • Cellular Imaging and Analysis Brochure

    The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.

Blocking Agents Selection Chart

NEB offers a number of blocking agents that block the reactivity of the SNAP-tag® or CLIP-tag™ intracellularly or on the surface of live cells. They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or CLIP-tag fusion proteins.

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.