Blocking agents are non-fluorescent compounds that block the reactivity of the SNAP- or CLIP-tag intracellularly (SNAP-Cell® Block, NEB #S9106; and CLIP-Cell™ Block, NEB #S9220) or on the surface of live cells (SNAP-Surface® Block, NEB #S9143; and CLIP-Cell Block). They can be used to generate inactive controls in live cell labeling experiments performed with SNAP- or CLIP-tag fusion proteins.
SNAP- and CLIP-Cell Block are highly membrane permeable and once in the cell react with the SNAP- or CLIP-tag, irreversibly inactivating it for subsequent labeling steps.
SNAP-Surface Block also reacts with the SNAP-tag irreversibly, inactivating it for subsequent labeling steps. This blocker is largely membrane impermeable essentially limiting blocking to cell surface-exposed SNAP-tags. CLIP-Cell Block may also be used to inactivate cell surface exposed CLIP-tags.
- Cellular Imaging & Analysis Brochure
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email firstname.lastname@example.org.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.