For optimal flexibility with existing technologies, biotinylated labels are available for studies using streptavidin platforms. Cell-permeable [SNAP-Biotin (NEB #S9110) and CLIP-Biotin (NEB #S9221)] or non-cell-permeable substrates [CoA-Biotin NEB #S9351] are suitable for applications such as biotinylation of fusion proteins in or on living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot. Biotin labels are also used for binding and protein interaction studies.
SNAP- and CLIP-Vista fluorescent substrates can be used to label SNAP- and CLIP-tag fusions in cell lysates or as purified proteins for detection by SDS-PAGE. The substrates are optimal for visualization using a standard UV-transilluminator [SNAP-Vista Blue NEB #S9146] or laser-based gel scanners [SNAP-Vista Green NEB #S9147; and CLIP-Vista Green NEB #S9235].
FAQs for Biotin/Vista Labels
Protocols for Biotin/Vista Labels
Cellular Imaging & Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
Other Tools & Resources
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.