Cellular Analysis

ACP/MCP-tag Substrates

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  • ACP/MCP-tag substrates are conjugated to the phosphopantetheinyl moeity of Coenzyme A (CoA) and require a separate enzyme (ACP or SFP Synthase) for labeling. The labeling reaction is specific for fusion proteins expressed on the cell surface. The same substrates are used to label both the ACP- and the MCP-tags; specificity is determined by the synthase used for labeling.

    1. Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

      Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

    2. SNAP-tag Overview Tutorial

      View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.

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