K. lactis Protease Deficient Competent Cell Sampler
- This product was discontinued on September 15, 2014
Chemically competent Kluyveromyces lactis cells are suitable hosts for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001S). Proteolysis of secreted heterologous proteins in K. lactis is most often casued by aspartyl poroteases that are present in the secretory pathway. In many cases, a single aspartyl protease causes the majority of the detrimental protein degredation. The Protease Deficient Competent Cell Sampler contains a wild-type GG799 strain, as well as four protease deficient K. lactis strains, each having selectable marker-free deletion of a specific secretory pathway aspartyl protease gene. Each strain can be transformed with any linearized pKLAC-series expression vector, permitting a comparison of heterologous proein quality and yield in each background.
The Sampler contains one transformation each of five K.lactis strains. K. lactis GG799 is a wild-type expression strain. K. lactis YCT389 contains a selectable marker-free deletion of the KLLA0E0398g locus encoding an extracellular aspartyl protease (KlYps1p) that is homologous to cerevisiae Yps1p. K. lactis YCT390 carries a selectable marker-free deletion of the KLLA0F2208g locus encoding an extracellular aspartyl protease (Klyps7p) that is homologous to S.cervisaiae Yps7p. K. lactis strain YCT 569 carries a selectable marker-free deletion of the KLLA0D01507g locus encoding an extracellular aspartyl protease belonging to pfam00026. K. lactis strain YCT598 carries a selectable marker-free deletion of the KLLA0D15917g locus encoding an extracellular aspartyl protease that is homologous to S.cervisaiae Bar1p.
The following reagents are supplied with this product:
Store at (°C) Concentration NEB® Yeast Transformation Reagent (5 ml) 4 1 X
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