OneTaq® (Mg-free) GC Reaction Buffer Pack

  • This product was discontinued on 01/05/2015
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Discontinued Products

Description

5X OneTaq (Mg-free) GC Reaction Buffer and OneTaq High GC Enhancer are optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer, along with supplemental enhancer and MgSO4 have been formulated for robust yields of difficult or GC-rich amplicons while allowing for maximal flexibility of Mg2+ optimization. The OneTaq (Mg-free) GC Reaction Buffer is recommended for GC-rich (>65% GC) amplicons. For particularly difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions with OneTaq (Mg-free) GC Reaction Buffer.  Supplemental MgSO4 is provided.


Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Magnesium Sulfate (MgSO4) Solution-20100 mM
OneTaq High GC Enhancer50%
OneTaq® (Mg-free) GC Reaction Buffer Pack-205X

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

80mM Tris-SO4
20mM (NH4)2SO4
5% Glycerol
5% DMSO
0.06% IGEPAL® CA-630
0.05% Tween® 20
pH 9.2@25°C

Notes

  1. OneTaq High GCEnhancer:
    10 mM Tris-HCl
    25% DMSO
    25% Glycerol
    pH 9.2 @25°C

FAQs

  1. When should I add the High GC Enhancer?
  2. Which buffer should I use if I want to control the level of magnesium in the reaction?
  3. The buffer arrived thawed. Are the buffer and enzyme still active?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • The reaction buffer and supplements are free of detectable nucleases.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.