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  • OneTaq® (Mg-Free) GC Reaction Buffer Pack


    5X OneTaq (Mg-Free) GC Reaction Buffer and OneTaq High GC Enhancer are optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer, along with supplemental enhancer and MgSO4 have been formulated for robust yields of difficult or GC-rich amplicons while allowing for maximal flexibility of Mg2+ optimization. The OneTaq (Mg-Free) GC Reaction Buffer is recommended for GC-rich (>65% GC) amplicons. For particularly difficult amplicons, the OneTaq High GC Enhancer can be added at a final concentration of 10–20% to reactions with OneTaq (Mg-Free) GC Reaction Buffer.  Supplemental MgSO4 is provided.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Magnesium Sulfate (MgSO4) Solution-20100 mM
    OneTaq High GC Enhancer100%
    OneTaq® (Mg-Free) GC Reaction Buffer Pack-205X

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    80mM Tris-SO4
    20mM (NH4)2SO4
    5% Glycerol
    5% DMSO
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    pH 9.2@25°C

    Quality Control

    Quality Assurance Statement

    • The reaction buffer and supplements are free of detectable nucleases.


    1. OneTaq High GC Enhancer:
      10 mM Tris-HCl
      25% DMSO
      25% Glycerol
      pH 9.2 @ 25°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. When should I add the High GC Enhancer?
    2. Which buffer should I use if I want to control the level of magnesium in the reaction?
    3. The buffer arrived thawed. Are the buffer and enzyme still active?

    Selection Tools

    Usage Guidelines & Tips