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  • OneTaq® (Mg-Free) Standard Reaction Buffer Pack


    5X OneTaq (Mg-Free) Standard Reaction Buffer is optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer has been formulated for robust yields while allowing for maximal flexibility in Mg2+ optimization. The OneTaq (Mg-Free) Standard Reaction Buffer with supplemental MgCl2 is recommended for most routine and/or AT-rich amplicons (i.e. Lambda, etc.) or complex amplicons with up to ~65% GC content that require complete control over the reaction's Mg2+ concentration. Supplemental MgCl2 is provided.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Magnesium Chloride (MgCl2) Solution-2025 mM
    OneTaq® (Mg-Free) Standard Reaction Buffer Pack-205X

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    22mM NH4Cl
    22mM KCl
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    pH 8.9@25°C

    Quality Control

    Quality Assurance Statement

    • The reaction buffer and supplement are free of detectable nucleases.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Which buffer should I use if I want to control the level of magnesium in the reaction?
    2. The buffer arrived thawed.  Are the buffer and enzyme still active?

    Selection Tools

    Usage Guidelines & Tips