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  • OneTaq® Standard Reaction Buffer Pack


    5X OneTaq Standard Reaction Buffer is optimized for use with OneTaq and OneTaq Hot Start DNA Polymerases. This reaction buffer has been formulated for robust yields with minimal optimization. The OneTaq Standard Reaction Buffer is recommended for most routine and/or AT-rich amplicons (i.e. Lambda, etc.) or complex amplicons with up to ~65% GC content.

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    22mM NH4Cl
    22mM KCl
    1.8mM MgCl2
    0.06% IGEPAL® CA-630
    0.05% Tween® 20
    pH 8.9@25°C

    Quality Control

    Quality Assurance Statement

    • The reaction buffer is free of detectable nucleases.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. The buffer arrived thawed. Are the buffer and enzyme still active?

    Selection Tools

    Usage Guidelines & Tips