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  • ThermoPol® DF (Detergent-free) Reaction Buffer Pack


    10X ThermoPol DF (Detergent-free) Reaction Buffer is optimized for use with Therminator™ and Taq DNA Polymerases in reactions which require a detergent-free buffer. This buffer also provides superior reaction conditions for other thermophilic DNA polymerases. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Magnesium Sulfate (MgSO4) Solution-20100 mM

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    10mM (NH4)2SO4
    10mM KCl
    2mM MgSO4
    pH 8.8@25°C

    Quality Control

    Quality Assurance Statement

    • This reaction buffer and supplement are free of detectable nucleases.


    1. 10X ThermoPol DF (Detergent-free) Reaction Buffer is identical to the standard 10X ThermoPol Reaction Buffer except for the absence of Triton X-100.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is supplied with the ThermoPol™ DF (detergent-free) Reaction Buffer pack?
    2. What is the composition of the ThermoPol™ DF (detergent-free) buffer?
    3. How should the NEBuffer be used?
    4. The buffer arrived thawed.  Are the buffer and the enzyme still active?