ThermoPol® II (Mg-free) Reaction Buffer Pack

Description

10X ThermoPol II (Mg-free) Reaction Buffer and supplemental MgSO4 are optimized for use with VentR® and Deep VentR™ DNA Polymerases. This buffer and supplement also provide superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes. The Mg-free reaction buffer and supplemental MgSO4 are provided for users that require complete control over Mg2+ concentration to optimize their specific application. See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Magnesium Sulfate (MgSO4) Solution-20100 mM
ThermoPol® II (Mg-free) Reaction Buffer Pack-2010X

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

20mM Tris-HCl
10mM (NH4)2SO4
10mM KCl
0.1% Triton® X-100
pH 8.8@25°C

FAQs

  1. What is supplied with the ThermoPol® II Reaction Buffer pack?
  2. What is the composition of the ThermoPol® II (Mg-free) Reaction Buffer?
  3. Which Polymerases use ThermoPol® II (Mg-free) Reaction Buffer?
  4. How should the NEBuffer™ be used?
  5. The buffer arrived thawed.  Are the buffer and enzyme still active?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Usage Guidelines & Tips

Interactive Tools

Quality Control

Quality Assurance Statement

  • This reaction buffer and supplement are free of detectable nucleases.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.