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  • BSA

    This product has been discontinued and replaced by NEB# B9000.

    Discontinued Date

    06/04/2013
    Catalog #SizeConcentrationPriceQtyAdd to Cart
      
    Categories:
    Discontinued Products

    Description

    Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    20 mM KPO4
    50 mM NaCl
    0.1 mM EDTA
    5% Glycerol
    pH 7.0 @ 25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
    • RNase Activity (1 Hour Digestion):
      The product is tested in a reaction containing a RNA substrate.  After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    Notes

    1. We do not recommend using this preparation of BSA as a standard for quantitation purposes or size determination.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. What is the source of your purified BSA?
    2. What is the buffer composition of your purified BSA?
    3. Will the buffer from the BSA inhibit my reaction?
    4. What quality controls are performed on your purified BSA?
    5. Why do I need to add BSA to my restriction digest?
    6. When setting up a restriction digest, if BSA is not listed as a reaction component, can it have a negative effect if added?
    7. Is the BSA in its native form?
    8. Can I use your BSA as a molecular weight marker?
    9. Can I use your BSA as a concentration standard?

    Usage Guidelines & Tips

    Troubleshooting Guides

    Interactive Tools