Red Loading Buffer Pack

An alternative product is Blue Loading Buffer (NEB #B7703)

  • Catalog # B7709 was discontinued on November 13, 2017 ;

The red loading buffer pack contains one vial of red loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.

  • 1X Red Loading Buffer Composition: 187.5 mM Tris-HCl (pH 7.0), 6% SDS (w/v), 30% glycerol, 0.03% phenol red (w/v)
  • 30X Reducing Agent: 1.25 M DTT
  • SDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shape 
  • DTT is present to help reduce structural disulfide bonds

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