NEBuffer Set (1, 2, 3, 4 & BSA)
- This product was discontinued on January 01, 2013
This NEBuffer minus BSA formulation is available for those enzymes requiring NEBuffers without BSA. For the BSA-containing formulation (which is included with NEB restriction enzymes), select NEBuffer Set 1.1, 2.1, 3.1 and CutSmart (NEB #B7200).
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. Some restriction endonucleases require BSA at a final concentration of 100 µg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture.
Restriction endonucleases that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction mixture.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 1 -20 10 X NEBuffer™ 2 -20 10 X NEBuffer™ 3 -20 10 X NEBuffer™ 4 -20 10 X Purified BSA -20 10 X
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