Luciferase Cell Lysis Buffer

  • Catalog # B3321 was discontinued on August 02, 2018 ;

Luciferase Cell Lysis Buffer is a proprietary formulation developed to produce mammalian cell lysates for reporter assays.

  • Compatible with reagents for assaying the activity of Gaussia, Cypridina and other luciferases as well as alkaline phosphatase and beta galactosidase
  • Cell lysates generated with LCLB can be used for western blots

Storage Temperature: 4°C

  • Product Information

    Luciferase Cell Lysis Buffer (LCLB) is a proprietary formulation developed to produce mammalian cell lysates for reporter assays. This lysis buffer is compatible with reagents for assaying the activity of Gaussia as well as other luciferases (e.g. Renilla & Firefly) and β-galactosidase (Figure 1A-D).

    Figure 1: LCLB compatibility with commonly used reporter assay systemsFigure 1: LCLB compatibility with commonly used reporter assay systems.
    NIH3T3 cells were transfected either with (A) pCMV-GLuc Control Plasmid (NEB #N8081S), (B) a lacZ-expressing vector, (C) a Renilla-expressing vector or (D) a Firefly-expressing vector, using TransPass D2 Transfection Reagent (NEB #M2554S). The supernatants from CMV-GLuc-transfected cells were saved for the assay at 24 h after transfection. All transfected cells were then washed once with PBS (pH 7.4) and lysed with either Luciferase Cell Lysis Buffer or commercially available lysis buffers recommended for each reporter system, respectively. Cell lysates (20 µL out of 100 µL total lysate per sample) were analyzed for GLuc or Renilla luciferase activity using the GLuc Assay Kit (NEB #E3300S/L), β-galactosidase activity using Galacto-Light System (ABI), and Firefly luciferase activity using Luciferase Assay System (Promega). Gaussia luciferase activity was also assayed from the supernatants (20 µL out of 500 µL growth medium of CMV-GLuc-transfected cells per sample).
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