Luciferase Cell Lysis Buffer

1. Depending on the cell type, some cells may need larger volume and longer incubation time for sufficient lysis. One can determine whether or not the lysis is sufficient by assaying the activity of intracellular Gaussia luciferase using the pCMV-GLuc Control Plasmid (NEB #N8081). Although the Gaussia luciferase produced from pCMV-GLuc is secreted into the culture medium, at a given time point, 5-10% of the total activity should reside in the lysate (Figure 1A).
2. Normally, there is no need to remove the cell debris by centrifugation before assaying Gaussia luciferase activity. If additional assays are to be performed (e.g. protein concentration, SDS-PAGE, etc.), it is best to centrifuge the cell lysate to remove cell debris.

1. Luciferase Cell Lysis Buffer

• Ultrasensitive Secreted Luciferases B3321

• Reporter Systems

1. What is the composition of the Luciferase Cell Lysis Buffer?
2. Can a lysis buffer other than the Luciferase Cell Lysis Buffer be used to make cell lysates for assaying GLuc (or CLuc) acitivity?
3. Can luciferases other than GLuc and CLuc be assayed from cell lysates made with the Luciferase Cell Lysis Buffer?
4. Are there differences between cell lines on how fast they can be lysed with the Luciferase Cell Lysis Buffer?
5. Do I need to remove cell debris in the cell lysate before assaying for GLuc or CLuc activity?

Product Citation Tool

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Luciferase Cell Lysis Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.