NEBuffer Pack for Protein MetalloPhosphatases (PMP)

Description

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer, supplemented with 1 mM MnCl2 assures optimal activity of PP1 (NEB #P0754) and Lambda Protein Phosphatase (Lambda PP NEB #P0753). At a 1X concentration, NEBuffer for PMP is suitable for other members of the metallophosphatases, after addition of an appropriate metal cation.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
MnCl210X
NEBuffer for PMP-2010X

Properties and Usage

Storage Temperature

-20°C

1X Buffer Components

50mM HEPES
100mM NaCl
2mM DTT
0.01% Brij 35
pH 7.5@25°C

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Protease Activity (SDS-PAGE):
    The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.