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  • Exoglycosidase Reaction Buffer Pack

    Description

    New England Biolabs supplies a 10X reaction buffer with all of its enzymes.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    G1 Reaction Buffer10X
    G2 Reaction Buffer10X
    G4 Reaction Buffer10X
    G6 Reaction Buffer10X
    G7 Reaction Buffer10X

    Properties and Usage

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • These buffers are free of detectable protease, exoglycosidase and endoglycosidase activities.

    Notes

    1. Buffer Compositions

      1X G1 Reaction Buffer:
      50 mM Sodium Citrate
      pH 6.0 @ 25°C

      1X G2 Reaction Buffer:
      50 mM Sodium Citrate
      pH 4.5 @ 25°C
       
      1X G4 Reaction Buffer:
      50 mM Sodium Citrate
      100 mM NaCl
      pH 6.0 @ 25°C
       
      1X G6 Reaction Buffer:
      5 mM CaCl2
      50 mM Sodium Acetate
      pH 5.5 @ 25°C

      1X G7 Reaction Buffer:
      50 mM Sodium Phosphate
      pH 7.5 @ 25°C

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Can I use α1-3,6 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?
    2. Can I use β-N-Acetylglucosaminidase in a double digest with other exoglycosidases and/or endoglycosidases?
    3. Can I use β1-4 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?