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  • NEBuffer I-SceI

    Discontinued Date

    01/01/2013
    Catalog #SizeConcentrationPriceQtyAdd to Cart
      
    Categories:
    Discontinued Products

    Description

    New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. Some restriction endonucleases require BSA at a final concentration of 100 µg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture.
    Restriction endonucleases that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction mixture.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    BSA-2010 mg/ml

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    10mM Tris-HCl
    10mM MgCl2
    1mM DTT
    pH 8.8@25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.
    1. What is supplied with the NEBuffer I-SceI pack?
    2. What is the compostion of NEBuffer I-SceI?
    3. How should the NEBuffer be used?
    4. The buffer arrived thawed.  Are the buffer and the enzyme still active?