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  • ProtoScript® II Reverse Transcriptase Reaction Buffer

    Description

    New England Biolabs supplies a 5X reaction buffer and 10X DTT with ProtoScript II Reverse Transcriptase. At a 1X concentration, this reaction buffer combination assures optimal activity of the enzyme.

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    ProtoScript® II Reverse Transcriptase Reaction Buffer-205X
    DTT (100mM)10X

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    50mM Tris-HCl
    75mM KCl
    3mM MgCl2
    pH 8.3@25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking, Buffer):
      The buffer is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Non-Specific DNase Activity (16 hour, DNA):
      The DNA is tested in a reaction under standard reaction conditions. After incubation for 16 hours there is no detectable degradation of the DNA as determined by agarose gel electrophoresis.
    • RNase Activity (Latent):
      The product is inactivated by heat treatment and tested in a reaction containing a RNA substrate. After incubationfor 2 hours there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.