Nuclease BAL-31 Reaction Buffer

  • This product was discontinued on 01/01/2013
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Discontinued Products


New England Biolabs supplies a reaction buffer with all of its enzymes. The buffer for Nuclease BAL-31 ensures optimal activity of the enzyme when used at a 1X concentration under the recommended reaction conditions described below.

Properties and Usage

Storage Temperature


1X Buffer Components

20mM Tris-HCl
600mM NaCl
12mM MgCl2
12mM CaCl2
pH 8.0@25°C

Interactive Tools

Quality Control

Quality Control Assays

The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here.
  • Endonuclease Activity (Nicking):
    The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
  • Non-Specific DNase Activity (16 hour):
    The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.