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  • Nuclease BAL-31 Reaction Buffer

    Discontinued Date

    01/01/2013
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    Categories:
    Discontinued Products

    Description

    New England Biolabs supplies a reaction buffer with all of its enzymes. The buffer for Nuclease BAL-31 ensures optimal activity of the enzyme when used at a 1X concentration under the recommended reaction conditions described below.

    Properties and Usage

    Storage Temperature

    -20°C

    1X Buffer Components

    20mM Tris-HCl
    600mM NaCl
    12mM MgCl2
    12mM CaCl2
    1mM EDTA
    pH 8.0@25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.