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  • Nuclease BAL-31 Reaction Buffer

    • This product was discontinued on 01/01/2013
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    Discontinued Products


    New England Biolabs supplies a reaction buffer with all of its enzymes. The buffer for Nuclease BAL-31 ensures optimal activity of the enzyme when used at a 1X concentration under the recommended reaction conditions described below.

    Properties and Usage

    Storage Temperature


    1X Buffer Components

    20mM Tris-HCl
    600mM NaCl
    12mM MgCl2
    12mM CaCl2
    1mM EDTA
    pH 8.0@25°C

    Interactive Tools

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Endonuclease Activity (Nicking):
      The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to the nicked form is determined by agarose gel electrophoresis.
    • Non-Specific DNase Activity (16 hour):
      The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16 hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.